Elucidation of roles for vitamin B<sub>12</sub> in regulation of folate, ubiquinone, and methionine metabolism [electronic resource]
Chemical Biology; Cobalamin; Metabolism; Microbial Regulation.
Saved in:
| Online Access: |
Online Access (via OSTI) |
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| Corporate Author: | |
| Format: | Government Document Electronic eBook |
| Language: | English |
| Published: |
Washington, D.C. : Oak Ridge, Tenn. :
United States. Department of Energy. ; distributed by the Office of Scientific and Technical Information, U.S. Department of Energy,
2017.
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| Subjects: |
| Summary: | Chemical Biology; Cobalamin; Metabolism; Microbial Regulation. |
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| Abstract: | Only a small fraction of vitamin B<sub>12</sub>-requiring organisms are able to synthesize B<sub>12</sub> de novo, making it a common commodity in microbial communities. Initially recognized as an enzyme cofactor of a few enzymes, recent studies have revealed additional B<sub>12</sub>-binding enzymes and regulatory roles for B<sub>12</sub>. Here we report the development and use of a B<sub>12</sub>-based chemical probe to identify B<sub>12</sub>-binding proteins in a nonphototrophic B<sub>12</sub>-producing bacterium. Two unexpected discoveries resulted from this study. First, we identified a new light-sensing B<sub>12</sub>-binding transcriptional regulator and demonstrated that it controls folate and ubiquinone biosynthesis. Second, our probe captured proteins involved in folate, methionine, and ubiquinone metabolism suggesting that it may play a role as an allosteric effector of these processes. These metabolic processes produce precursors for synthesis of DNA, RNA, and protein. Furthermore, B<sub>12</sub> modulates growth, and by limiting its availability to auxotrophs, B<sub>12</sub>-producing organisms may facilitate coordination of community metabolism. |
| Item Description: | Published through SciTech Connect. 01/30/2017. "pnnl-sa--119181" "KP1601010" Proceedings of the National Academy of Sciences of the United States of America 114 7 ISSN 0027-8424 AM. Margaret F. Romine; Dmitry A. Rodionov; Yukari Maezato; Lindsey N. Anderson; Premchendar Nandhikonda; Irina A. Rodionova; Alexandre Carre; Xiaoqing Li; Chengdong Xu; Therese R. W. Clauss; Young -Mo Kim; Thomas O. Metz; Aaron T. Wright. |
| Physical Description: | p. E1205-E1214 : digital, PDF file. |