A 5-enolpyruvylshikimate 3-phosphate synthase functions as a transcriptional repressor in Populus [electronic resource]
Poplar; Lignin Biosynthesis; Transcriptional Repressors; Populus.
Saved in:
| Online Access: |
Online Access (via OSTI) |
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| Corporate Author: | |
| Format: | Government Document Electronic eBook |
| Language: | English |
| Published: |
Washington, D.C. : Oak Ridge, Tenn. :
United States. Department of Energy. Office of Science ; distributed by the Office of Scientific and Technical Information, U.S. Department of Energy,
2018.
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| Subjects: |
MARC
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| 245 | 0 | 2 | |a A 5-enolpyruvylshikimate 3-phosphate synthase functions as a transcriptional repressor in Populus |h [electronic resource] |
| 260 | |a Washington, D.C. : |b United States. Department of Energy. Office of Science ; |a Oak Ridge, Tenn. : |b distributed by the Office of Scientific and Technical Information, U.S. Department of Energy, |c 2018. | ||
| 300 | |a p. 1645-1660 : |b digital, PDF file. | ||
| 336 | |a text |b txt |2 rdacontent. | ||
| 337 | |a computer |b c |2 rdamedia. | ||
| 338 | |a online resource |b cr |2 rdacarrier. | ||
| 500 | |a Published through SciTech Connect. | ||
| 500 | |a 06/11/2018. | ||
| 500 | |a "nrel/ja-2700-71648" | ||
| 500 | |a Plant Cell 30 7 ISSN 1040-4651 AM. | ||
| 500 | |a Meng Xie; Wellington Muchero; Anthony C. Bryan; Kelsey L. Yee; Hao-bo Guo; Jin Zhang; Timothy Tschaplinski; Vasanth R. Singan; Erika Lindquist; Raja S. Payyavula; Jaime Barros-Rios; Richard A. Dixon; Nancy Engle; Robert W. Sykes; Mark Davis; Sara Jawdy; Lee E. Gunter; Olivia Thompson; Stephen P. DiFazio; Luke M. Evans; Kim Winkler; Cassandra Collins; Jeremy Schmutz; Hong Guo; Udaya Kalluri; Miguel Rodriguez; Kai Feng; Jin-Gui Chen; Gerald A. Tuskan. | ||
| 520 | 3 | |a Long-lived perennial plants, with distinctive habits of inter-annual growth, defense, and physiology, are of great economic and ecological importance. However, some biological mechanisms resulting from genome duplication and functional divergence of genes in these systems remain poorly studied. Here, we discovered an association between a poplar (Populus trichocarpa) 5-enolpyruvylshikimate 3-phosphate synthase gene (PtrEPSP) and lignin biosynthesis. Functional characterization of PtrEPSP revealed that this isoform possesses a helix-turn-helix motif in the N-terminus and can function as a transcriptional repressor that regulates expression of genes in the phenylpropanoid pathway in addition to performing its canonical biosynthesis function in the shikimate pathway. We demonstrated that this isoform can localize in the nucleus and specifically binds to the promoter and represses the expression of a SLEEPER-like transcriptional regulator, which itself specifically binds to the promoter and represses the expression of PtrMYB021 (known as MYB46 in Arabidopsis thaliana), a master regulator of the phenylpropanoid pathway and lignin biosynthesis. Analyses of overexpression and RNAi lines targeting PtrEPSP confirmed the predicted changes in PtrMYB021 expression patterns. These results demonstrate that PtrEPSP in its regulatory form and PtrhAT form a transcriptional hierarchy regulating phenylpropanoid pathway and lignin biosynthesis in Populus. | |
| 520 | 0 | |a Poplar; Lignin Biosynthesis; Transcriptional Repressors; Populus. | |
| 536 | |b AC36-08GO28308. | ||
| 536 | |b AC05-00OR22725. | ||
| 650 | 7 | |a Biomass Fuels. |2 edbsc. | |
| 710 | 2 | |a National Renewable Energy Laboratory (U.S.). |4 res. | |
| 710 | 1 | |a United States. |b Department of Energy. |b Office of Science. |4 spn. | |
| 710 | 1 | |a United States. |b Department of Energy. |b Office of Scientific and Technical Information. |4 dst. | |
| 856 | 4 | 0 | |u http://www.osti.gov/scitech/biblio/1457659 |z Online Access (via OSTI) |
| 907 | |a .b104857468 |b 03-09-23 |c 05-17-19 | ||
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| 952 | f | f | |p Can circulate |a University of Colorado Boulder |b Online |c Online |d Online |e E 1.99:nrel/ja-2700-71648 |h Superintendent of Documents classification |i web |n 1 |