|
|
|
|
| LEADER |
00000nam a22000003u 4500 |
| 001 |
b10786302 |
| 003 |
CoU |
| 005 |
20190619223611.9 |
| 006 |
m o d f |
| 007 |
cr ||||||||||| |
| 008 |
191002e20180618||| o| f0|||||eng|d |
| 035 |
|
|
|a (TOE)ost1461176
|
| 035 |
|
|
|a (TOE)1461176
|
| 040 |
|
|
|a TOE
|c TOE
|
| 049 |
|
|
|a GDWR
|
| 072 |
|
7 |
|a 59
|2 edbsc
|
| 086 |
0 |
|
|a E 1.99:1461176
|
| 086 |
0 |
|
|a E 1.99:1461176
|
| 245 |
0 |
0 |
|a <em>De novo</em> DNA synthesis using polymerase-nucleotide conjugates
|h [electronic resource]
|
| 260 |
|
|
|a Washington, D.C. :
|b United States. Department of Energy. Office of Science ;
|a Oak Ridge, Tenn. :
|b distributed by the Office of Scientific and Technical Information, U.S. Department of Energy,
|c 2018.
|
| 300 |
|
|
|a p. 645-650 :
|b digital, PDF file.
|
| 336 |
|
|
|a text
|b txt
|2 rdacontent.
|
| 337 |
|
|
|a computer
|b c
|2 rdamedia.
|
| 338 |
|
|
|a online resource
|b cr
|2 rdacarrier.
|
| 500 |
|
|
|a Published through SciTech Connect.
|
| 500 |
|
|
|a 06/18/2018.
|
| 500 |
|
|
|a "ark:/13030/qt9d4221mc"
|
| 500 |
|
|
|a Nature Biotechnology 36 7 ISSN 1087-0156 AM.
|
| 500 |
|
|
|a Sebastian Palluk; Daniel H. Arlow; Tristan de Rond; Sebastian Barthel; Justine S. Kang; Rathin Bector; Hratch M. Baghdassarian; Alisa N. Truong; Peter W. Kim; Anup K. Singh; Nathan J. Hillson; Jay D. Keasling.
|
| 520 |
3 |
|
|a Oligonucleotides are almost exclusively synthesized using the nucleoside phosphoramidite method, even though it is limited to the direct synthesis of ̃200 mers and produces hazardous waste. Here, we describe an oligonucleotide synthesis strategy that uses the template-independent polymerase terminal deoxynucleotidyl transferase (TdT). Each TdT molecule is conjugated to a single deoxyribonucleoside triphosphate (dNTP) molecule that it can incorporate into a primer. After incorporation of the tethered dNTP, the 3' end of the primer remains covalently bound to TdT and is inaccessible to other TdT-dNTP molecules. Cleaving the linkage between TdT and the incorporated nucleotide releases the primer and allows subsequent extension. We demonstrate that TdT-dNTP conjugates can quantitatively extend a primer by a single nucleotide in 10-20 s, and that the scheme can be iterated to write a defined sequence. Furthermore, this approach may form the basis of an enzymatic oligonucleotide synthesizer.
|
| 536 |
|
|
|b AC02-05CH11231.
|
| 650 |
|
7 |
|a Basic Biological Sciences.
|2 edbsc.
|
| 710 |
2 |
|
|a Lawrence Berkeley National Laboratory.
|4 res.
|
| 710 |
1 |
|
|a United States.
|b Department of Energy.
|b Office of Science.
|4 spn.
|
| 710 |
1 |
|
|a United States.
|b Department of Energy.
|b Office of Scientific and Technical Information.
|4 dst.
|
| 856 |
4 |
0 |
|u http://www.osti.gov/scitech/biblio/1461176
|z Full Text (via OSTI)
|
| 907 |
|
|
|a .b107863029
|b 10-03-19
|c 10-03-19
|
| 998 |
|
|
|a web
|b 10-03-19
|c f
|d m
|e p
|f eng
|g
|h 0
|i 1
|
| 956 |
|
|
|a Information bridge
|
| 999 |
f |
f |
|i 3112e114-f160-5a53-9b10-b53df4a19041
|s 9c5ec3d7-cfba-524a-9848-ffa0c2d69f53
|
| 952 |
f |
f |
|p Can circulate
|a University of Colorado Boulder
|b Online
|c Online
|d Online
|e E 1.99:1461176
|h Superintendent of Documents classification
|i web
|n 1
|
