Simple measurements in a complex system [electronic resource] : soil community responses to nitrogen amendment in a <em>Pinus taeda</em> forest.
Biological Science; Carbon Use Efficiency; Extracellular Ecoenzymes; N Amendment; Ribosomal Rna Genes; Soil Bacterial Community; Soil Carbon Cycling; Soil Fungal Community; Soil Microbiome; Temperate Pine Forest.
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Full Text (via OSTI) |
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| Format: | Government Document Electronic eBook |
| Language: | English |
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Washington, D.C. : Oak Ridge, Tenn. :
United States. Department of Energy. Office of Science ; distributed by the Office of Scientific and Technical Information, U.S. Department of Energy,
2019.
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| 245 | 0 | 0 | |a Simple measurements in a complex system |h [electronic resource] : |b soil community responses to nitrogen amendment in a <em>Pinus taeda</em> forest. |
| 260 | |a Washington, D.C. : |b United States. Department of Energy. Office of Science ; |a Oak Ridge, Tenn. : |b distributed by the Office of Scientific and Technical Information, U.S. Department of Energy, |c 2019. | ||
| 300 | |a Article No. e02687 : |b digital, PDF file. | ||
| 336 | |a text |b txt |2 rdacontent. | ||
| 337 | |a computer |b c |2 rdamedia. | ||
| 338 | |a online resource |b cr |2 rdacarrier. | ||
| 500 | |a Published through SciTech Connect. | ||
| 500 | |a 04/05/2019. | ||
| 500 | |a "la-ur--18-23932" | ||
| 500 | |a Ecosphere 10 4 ISSN 2150-8925 AM. | ||
| 500 | |a Cheryl Rae Kuske; Robert L. Sinsabaugh; La Verne Gallegos‐Graves; Michaeline Burr Nelson Albright; Rebecca Mueller; John Martin Dunbar. | ||
| 520 | 3 | |a Microorganisms regulate the decomposition of soil organic matter and the flow of plant-available nutrients. In a temperate pine forest in North Carolina, USA, where nitrogen (N) had been experimentally added for eight years, we combined DNA-based measures of fungal and bacterial biomass and composition with measures of seven extracellular enzymes (ecoenzymes). These measures were then correlated with soil chemistry. Our goals were to evaluate the relative sensitivity and repeatability of multiple structural and functional measures of community organization to long-term N deposition. Measurements were conducted at three litter/soil depths, corresponding to the litter, Oa, and mineral A horizons (max depth 10 cm) with ten spatial replicates per experimental plot. Soil chemistry differed significantly with soil depth and N amendment. Total biomass (soil DNA), as well as fungal and bacterial biomass (measured by qPCR), was greatest in the litter horizon and declined significantly with depth. Ecoenzyme activity patterns also changed with soil depth, transitioning from high levels of C, N, and P metabolizing activities in the litter horizon to increased oxidative activities at the lower depth. Under N amendment, soil pH decreased and nitrate concentrations increased in all three soil horizons. Correspondingly, the estimated microbial C use efficiency decreased in N-amended soils at all depths, despite differences in microbial biomass, community composition, and soil chemistry. Overall, bacterial composition was most responsive to nitrogen amendment, but the taxonomic context of the response varied with soil horizons in conjunction with shifts in soil chemistry and enzyme activities. While a single measure that would incorporate all of the C, N, and P metabolizing activities did not emerge, many measures correlated with each other, and/or with depth and/or N amendment. | |
| 520 | 0 | |a Biological Science; Carbon Use Efficiency; Extracellular Ecoenzymes; N Amendment; Ribosomal Rna Genes; Soil Bacterial Community; Soil Carbon Cycling; Soil Fungal Community; Soil Microbiome; Temperate Pine Forest. | |
| 536 | |b 89233218CNA000001. | ||
| 710 | 2 | |a Los Alamos National Laboratory. |4 res. | |
| 710 | 1 | |a United States. |b Department of Energy. |b Office of Science. |4 spn. | |
| 710 | 1 | |a United States. |b Department of Energy. |b Office of Scientific and Technical Information. |4 dst. | |
| 856 | 4 | 0 | |u http://www.osti.gov/scitech/biblio/1525839 |z Full Text (via OSTI) |
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