Gene silencing by RNA interference : technology and application / edited by Muhammad Sohail.
Maximizing the potential of RNA interference in functional genomics - as well as in the development of therapeutics - continues to be at the forefront of biomedical research. Unlike journal articles, Gene Silencing by RNA Interference: Technology and Application combines essential background to the...
Saved in:
| Online Access: |
Full Text (via Taylor & Francis) |
|---|---|
| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
Boca Raton, FL :
CRC Press,
©2005.
|
| Subjects: |
Table of Contents:
- Gene silencing by RNA interference and the role of small interfering RNAs
- Basics of siRNA design and chemical synthesis
- Oligonucleotide scanning arrays in the design of small interfering RNAs
- siRNA production by in vitro transcription
- Production of siRNAs with the application of deoxyribozymes
- Production of siRNA in vitro by enzymatic digestion of double-stranded RNA
- Plasmid-mediated intracellular expression of siRNAs
- Lentiviral vector-mediated delivery of si/shRNA
- Exogenous siRNA delivery: protocols for optimizing delivery to cells
- RNAi in drosophila cell cultures
- RNAi in caenorhabditis elegans
- Delivery of RNAi reagents in C. elegans by microinjection
- RNAi in drosophila embryos
- RNAi in xenopus laevis oocytes
- RNAi in xenopus embryos
- An RNAi-based genomic library for forward genetics in the African trypanosome
- RNAi in the malaria parasite plasmodium
- Silencing the expression of cysteine proteases (falcipains) in plasmodium falciparum using RNA interference approaches
- RNAi in transgenic animal models
- RNA interference in mouse models
- Virus-induced RNA silencing in plants
- RNA silencing in plants: biolistic delivery of RNAi reagents
- Agro-infiltration: a versatile tool for RNAi studies in plants.