Producing biomolecular substances with fermenters, bioreactors, and biomolecular synthesizers [electronic resource] / William L. Hochfeld.
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| Online Access: |
Full Text (via Taylor & Francis) |
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| Main Author: | |
| Other title: | BIOSCIENCEnetBASE. |
| Format: | Electronic eBook |
| Language: | English |
| Published: |
Boca Raton, FL :
CRC/Taylor & Francis,
2006.
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| Subjects: |
Table of Contents:
- Introduction ; Biotechnology; Bioproduction; The Bioprocess; Cell Lines; Cell-Culture Process; Separation, Recovery,
- Purification; Virus and Foreign-DNA Removal; Quality Assurance; Technology Overview ; Biomolecular Foundations; Cellular Variation; Key Molecular Interactions; Base-Pair Complementarity; Genetic Coding; Interrupting Gene Segments; Sequence Determinations; Cloning; Synthesized DNA; Cell Lines; Expression Systems Introduction to the Bioprocess ; Overview; Optimizing Product Yield; Kinetic Models; cGMP Standards; Contract Manufacturing; Complex Mixtures; Biomolecular Products; Joint Manufacturing; Downscaling to Scale Up; Large-Scale Bioprocesses; Scaling-Up In-House or in a Contract Facility; Lab-to-Pilot-Plant-to-Production; Increasing Bioprocess Scale; Differing Features at Larger Scale; Simulating Environment at Scale; Detailed Specifications; Impact on Downstream Bioprocessing; FDA Biologics Review Process.
- ; Regulating Biological Products Recombinant DNA Materials and Methods ; Cloning; Applications; Screening and Selection; DNA Synthesis; Cell Lines; Expression Systems; Vector Construction; Prokaryotic and Eukaryotic Growth Characteristics; Expression Levels; Intra- vs.
- Extra-cellular Expression; Intracellular Expression; Extracellular Expression; Glycosylation; Mammalian Cell Glycosylation; Insect Cell Glycosylation; General Considerations; Further Post-translational Modifications; Economic Concerns; Regulatory Concerns; Manufacturers' Directory Enzymes ; Overview; Enzyme Protein Engineering; Transformation in Non-Aqueous Systems; Restriction Enzymes; Restriction-Enzyme Classes; Class I Restriction Enzymes; Class II Restriction Enzymes; Class III Restriction Enzymes; Restriction-Enzyme Specificity; Star Activity; Enzyme Families and Compatible Ends; Isoschizomers; Reverse Transcriptases; DNA and RNA Nucleases; Manufacturers' Directory DNA-Amplification Reagents ; Overview; Amplification by DNA Synthesis (PCR); Amplification by RNA Transcription (TAS and 3SR; Amplification by Ligation (LAR, LCR,
- LAS); Amplification by RNA Replication (Q Replicase); Sequence-Based Amplification; Manufacturers Directory Cell-Culture Media ; Overview; Prokaryotic Cell-Culture Medium; Eukaryotic Cell-Culture Medium; Defined Medium; Serum; Other Sera; Serum Contamination; Serum-Free (Defined) Medium; Cell-Culture Gels; Media Supplements; Attachment Factors; Buffers; Sodium; Earle's Balanced Salt Solution; Growth Factors; Epidermal Growth Factor (EGF); Fibroblast Growth Factors (FGF); Acidic FGF; Basic FGF; Other Growth Factors; Lectins; Toxins; Transport Factors; Vitamins; Water; Manufacturers' Directory Fermenters ; Introduction to Fermentation; Historical Perspective; The Fermentation Process; Enzyme Fermentation in the Organic Phase; Cell Growth and Production; Factors Affecting Specific Growth Rate; Kinetic.
- Models; Optimal Conditions; Recombinant Culture Kinetics; Fermenter Design; Kinetic Modeling of the Fermentation Process; STF Batch Scale Up; Power Number Method for STF Geometric Scale Up; Continuous-Culture Scale Up; Fermenter Manufacturers Directory Bioreactors ; Introduction; Aseptic Conditions; Monoclonal Antibody Production; mAB Production in Ascites Fluid; Bioreactor Engineering and Design; Bioreactors II: Kinetic Modeling and Bioreactor Dynamics and Bioreactor Design Program; Bioreactor Manufacturers Directory Biomolecular Synthesizers ; DNA Synthesizers; Manufacturers Directory; Technology Overview; Peptides by Solid-Phase Synthesis; Manufacturers Directory; Biotechnology Glossary ; References and Recommended Reading Index.