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|a (TOE)ost1783232
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|a Post-Synthetic Reduction of Pectin Methylesterification Causes Morphological Abnormalities and Alterations to Stress Response in Arabidopsis thaliana
|h [electronic resource]
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|a Oak Ridge, Tenn. :
|b Distributed by the Office of Scientific and Technical Information, U.S. Department of Energy,
|c 2020.
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| 300 |
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|a Size: Article No. 1558 :
|b digital, PDF file.
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|a text
|b txt
|2 rdacontent.
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|a online resource
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|a Published through Scitech Connect.
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|a 11/12/2020.
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|a "is-j-10,480."
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|a "Journal ID: ISSN 2223-7747."
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|a Reem, Nathan T. ; Chambers, Lauran ; Zhang, Ning ; Abdullah, Siti Farah ; Chen, Yintong ; Feng, Guanhua ; Gao, Song ; Soto-Burgos, Junmarie ; Pogorelko, Gennady ; Bassham, Diane C. ; et al;
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|a Ames Lab., Ames, IA (United States)
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|a USDOE.
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|a U.S. Department of Agriculture-National Institute of Food and Agriculture.
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|a Pectin is a critical component of the plant cell wall, supporting wall biomechanics and contributing to cell wall signaling in response to stress. The plant cell carefully regulates pectin methylesterification with endogenous pectin methylesterases (PMEs) and their inhibitors (PMEIs) to promote growth and protect against pathogens. We expressed Aspergillus nidulans pectin methylesterase (AnPME) in Arabidopsis thaliana plants to determine the impacts of methylesterification status on pectin function. Plants expressing AnPME had a roughly 50% reduction in methylester content compared with control plants. AnPME plants displayed a severe dwarf phenotype, including small, bushy rosettes and shorter roots. This phenotype was caused by a reduction in cell elongation. Cell wall composition was altered in AnPME plants, with significantly more arabinose and significantly less galacturonic acid, suggesting that plants actively monitor and compensate for altered pectin content. Cell walls of AnPME plants were more readily degraded by polygalacturonase (PG) alone but were less susceptible to treatment with a mixture of PG and PME. AnPME plants were insensitive to osmotic stress, and their susceptibility to Botrytis cinerea was comparable to wild type plants despite their compromised cell walls. This is likely due to upregulated expression of defense response genes observed in AnPME plants. These results demonstrate the importance of pectin in both normal growth and development, and in response to biotic and abiotic stresses.
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|b AC02-07CH11358.
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|b 015227-00005.
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|b MCB-1616316.
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|a 59 basic biological sciences
|2 local.
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|a National Science Foundation (U.S.).
|4 spn.
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|a United States.
|b Department of Energy.
|b Office of Scientific and Technical Information
|4 dst.
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|u https://www.osti.gov/servlets/purl/1783232
|z Full Text (via OSTI)
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|a .b11871708x
|b 06-23-21
|c 06-23-21
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|a University of Colorado Boulder
|b Online
|c Online
|d Online
|e E 1.99:is-j-10,480
|h Superintendent of Documents classification
|i web
|n 1
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