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|a (TOE)ost1830103
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|a E 1.99:1830103
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|a E 1.99:1830103
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|a Characterization and structural analysis of a thermophilic GH11 xylanase from compost metatranscriptome
|h [electronic resource]
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|a Oak Ridge, Tenn. :
|b Oak Ridge National Laboratory ;
|a Oak Ridge, Tenn. :
|b Distributed by the Office of Scientific and Technical Information, U.S. Department of Energy,
|c 2021.
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| 300 |
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|a Size: p. 7757-7767 :
|b digital, PDF file.
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|a text
|b txt
|2 rdacontent.
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|a online resource
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|a Published through Scitech Connect.
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|a 09/23/2021.
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|a "Journal ID: ISSN 0175-7598."
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|a Yi, Yunlei ; Xu, Shenyuan ; Kovalevsky, Andrey ; Zhang, Xia ; Liu, Dongyang ; Wan, Qun ;
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|a USDOE.
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|a Fundamental Research Funds for the Central Universities.
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|a Xylanase is efficient for xylan degradation and widely applied in industries. We found a GH11 family xylanase (Xyn11A) with high thermostability and catalytic activity from compost metatranscriptome. This xylanase has the optimal reaction temperature at 80 ʻC with the activity of 2907.3 U/mg. The X-ray crystallographic structure shows a typical ?right hand? architecture, which is the characteristics of the GH11 family enzymes. Comparing it with the mesophilic XYN II, a well-studied GH11 xylanase from Trichoderma reesei, Xyn11A is more compact with more H-bonds. Our mutagenic results show that the electrostatic interactions in the thumb and palm region of Xyn11A could result in its high thermostability and activity. Introducing a disulfide bond at the N-terminus further increased its optimal reaction temperature to 90 ʻC with augmented activity.
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|b AC05-00OR22725.
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|b 31670790.
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|b KYXK202009.
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|a 59 basic biological sciences
|2 local.
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|a Gh11 xylanase
|2 local.
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|a Thermostability
|2 local.
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|a Crystal structure
|2 local.
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|a Md simulations
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|a Basic biological sciences
|2 local.
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|a Oak Ridge National Laboratory.
|4 res.
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|a National Natural Science Foundation of China (NSFC).
|4 spn.
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|a United States.
|b Department of Energy.
|b Office of Scientific and Technical Information
|4 dst.
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|u https://www.osti.gov/servlets/purl/1830103
|z Full Text (via OSTI)
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|a .b12823958x
|b 02-28-23
|c 12-08-22
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|b 12-08-22
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|p Can circulate
|a University of Colorado Boulder
|b Online
|c Online
|d Online
|e E 1.99:1830103
|h Superintendent of Documents classification
|i web
|n 1
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