PCR sequencing protocols / edited by Ralph Rapley.
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| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
©1996.
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| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 65. |
| Subjects: |
Table of Contents:
- Preparation and analysis of DNA sequencing gels
- Purification of PCR products from agarose gels for direct sequencing
- Enzymatic fluorescence and biotin labeling of primers for PCR sequencing
- Direct sequencing of double-stranded PCR products with the sequenase kit and [alpha-35S] dATP
- Direct sequencing by thermal asymmetric PCR
- Rapid sequencing of cDNA clones: direct sequencing using sequential linear/asymmetric PCR
- Direct sequencing of PCR products using chemiluminescent detection
- Direct DNA sequencing of PCR products using magnetic beads
- Affinity capture and solid-phase sequencing of biotinylated PCR products
- Analysis of nucleotide sequence variations by solid-phase minisequencing
- Nonradioactive PCR sequencing using digoxigenin
- Silver Sequencing(TM) nonradioactive cycle sequencing of dsDNA
- Direct sequencing of PCR products with DNA-binding proteins
- PCR sequencing with the aid of detergents
- Direct sequencing with highly degenerate and inosine-containing primers
- Determination of unknown genomic sequences without cloning
- DNA sequencing by the chemical method
- Direct PCR sequencing with denaturants (formamide)
- Efficient PCR production of single-stranded DNA sequencing templates
- Preparation and direct automated cycle sequencing of PCR products
- Solid-phase automated sequencing of PCR-ampilified genomic DNA
- Cloning PCR products for sequencing in M13 vectors
- Sequencing PCR products cloned into M13 vectors
- Genomic ampilificatin with transcript sequencing (GAWTS)
- DNA rescue by the vectorette method
- Sequencing of (dA:dT) cloned mixed PCR products from microbial populations
- Index.