Bacterial toxins : methods and protocols / edited by Otto Holst.
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| Other Authors: | |
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| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
©2000.
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| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 145. |
| Subjects: |
Table of Contents:
- Bacterial protein toxins: an overview
- Purification of clostridium botulinum type A neurotoxin
- Shiga toxins
- Isolation and detection of microcystins and nodularins, cyanobacterial peptide hepatotoxins
- Genetic construction, expression, and characterization of diphtheria toxin-based growth factor fusion proteins
- Structure-function analysis of cysteine-engineered entomopathogenic toxins
- Use of fourier-transformed infrared spectroscopy for secondary structrue determination of staphyloccoccal pore-forming toxins
- The use of fluorescence resonance energy transfer to detect conformational changes in protein toxins
- Site-directed spin labeling of proteins: applications to diphtheria toxin
- Characterization of molecular properties of pore-forming toxins with planar lipid bilayers
- Determination of affinity and kinetic rate constants using surface plasmon resonance
- ADP-ribosylation of α-Gi proteins by pertusis toxin: positional dissection of acceptor sites using membrane anchored synthetic peptides
- Phage libraries for generation of anti-botulinum scFv antibiodies
- T-cell cytotoxicity assays for studing the functional itneraction between the superantigen staphylococcal enterotoxin A and T-cell receptors
- In vitro physiological studies on clostridial neurotoxins: biological modesl and procedures for extracellular and intracellular application of toxins
- The biology of endotoxin
- Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of lipopolysaccharides
- Applications of combined capillary electrophoresis-electrospray mass spectrometry in the characterization of short-chain lipopolysaccharides: haemophilus influenzae
- Deacylation of lipopolysaccharides and isolation of oligosaccharide phosphates
- Electrophysiological measurement on reconstituted outer membranes.