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Handbook of ELISPOT : methods and protocols / edited by Alexander E. Kalyuzhny.

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Bibliographic Details
Other Authors: Kalyuzhny, Alexander E.
Format: Book
Language:English
Published: Totowa, N.J. : Humana Press, ©2005.
Series:Methods in molecular biology (Clifton, N.J.) ; v. 302.
Subjects:
Enzyme-linked immunosorbent assay.
Enzyme-Linked Immunosorbent Assay > methods.
Cytokines > immunology.
Interferon Type II > immunology.
T-Lymphocytes > immunology.
Table of Contents:
  • The ELISPOT assay : a personal retrospective / Jonathan Sedgwick
  • Chemistry and biology of the ELISPOT assay / Alexander E. Kalyuzhny
  • Membranes and membrane plates used in ELISPOT / Alan J. Weiss
  • Standardization and validation issues of the ELISPOT assay / Sylvia Janetzki ... [et al.]
  • A cell detachment solution can reduce a background staining in ELISPOT assay : Accumax / Angela Grant ... [et al.]
  • Isolation of subsets of immune cells / Carrie E. Peters, Steven M. Woodside, and Allen C. Eaves
  • Image analysis and data management of ELISPOT assay results / Paul Viktor Lehmann
  • High resolution as a key feature to perform accurate ELISPOT measurements using Zeiss KS ELISPOT readers / Wolf Malkusch
  • Improving the sensitivity of the ELISPOT analyses of antigen-specific cellular immune responses in Rhesus macaques / K. Jagannadha Sastry, Pramod N. Nehete, and Bharti Nehete
  • Feline cytokine ELISPOT : issues in assay development / Sushila K. Nordone ... [et al.]
  • Measuring T cell function in animal models of tuberculosis by ELISPOT / Vanja Lazarevic, Santosh Pawar, and JoAnne Flynn
  • IFN-gamma ELISPOT assay for the quantitative measurement of antigen-specific murine CD8+ T cells / Geoffrey A. Cole
  • Detection of measles virus-specific IFN-gamma secreting T cells by ELISPOT / Jenna E. Ryan, Inna G. Ovsyannikova, and Gregory A. Poland
  • Epitope mapping in multiple sclerosis using the ELISPOT assay / Clara M. Pelfrey and Ioana R. Moldovan
  • Use of IFN-gamma ELISPOT in monitoring immune responses in humans / Mark Matijevic and Robert G. Urban
  • ELISPOT determination of IFN-gamma T cell frequencies in patients with autoimmune sensorineural hearing loss / C. Arturo Solares and Vincent K. Tuohy
  • Dual color ELISPOT assay for analyzing cytokine balance / Yoshihiro Okamoto and Mikio Nishida
  • Simultaneous detection of multiple cytokines in ELISPOT assays / Sarah Palzer ... [et al.]
  • Fluorospot assay : methodological analysis / Agnes Gazagne ... [et al.]
  • A gel-based dual antibody capture and detection method for assaying of extracellular cytokine secretion : EliCell / Lisa A. Spencer ... [et al.]

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