Applications of optical trapping to single molecule DNA [electronic resource]
Saved in:
| Online Access: |
Online Access |
|---|---|
| Corporate Author: | |
| Format: | Government Document Electronic eBook |
| Language: | English |
| Published: |
Washington, DC : Oak Ridge, Tenn. :
United States. Dept. of Energy. Assistant Secretary for Human Resources and Administration ; distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy,
1997.
|
| Subjects: |
| Abstract: | This is the final report of a three-year, Laboratory Directed Research and Development (LDRD) project at the Los Alamos National Laboratory (LANL). The project focused on the methodologies required to integrate optical trapping with single molecule detection (SMD) so as to demonstrate high speed sequencing through optical micromanipulation of host substrates, nucleotide cleavage, and single molecule detection. As part of this effort, the new technology of optical tweezers was applied to the confinement and manipulation of microsphere handles containing attached DNA fragments. The authors demonstrated substrate optical trapping in rapid flow streams, the fluorescence excitation and detection of fluorescently labeled nucleotides in an optical trapping system, and the epifluorescent imaging of DNA fragments in flow streams. They successfully demonstrated optical trapping in laminar flow streams and completely characterized the trapping process as functions of fluid flow velocity, chamber dimension, trapping depth, incident laser power, and fluorescence measurement geometry. |
|---|---|
| Item Description: | Published through the Information Bridge: DOE Scientific and Technical Information. 12/01/1997. "LA-UR--97-3253" "DE98001512" Keller, R.A.; Berns, M.W.; Sonek, G.J. |
| Physical Description: | 6 p. |