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|a (TOE)ost10166528
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|a (TOE)10166528
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|a TOE
|c TOE
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|a GDWR
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|a E 1.99:doe/er/13546--4
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|a E 1.99:doe/er/13546--4
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| 245 |
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|a [Organization and regulation of the genes for nitrogen fixation in Rhodopseudomonas capsulata]. Progress report, [June 5, 1989--June 4, 1991]
|h [electronic resource]
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| 260 |
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|a Washington, D.C. :
|b United States. Dept. of Energy ;
|a Oak Ridge, Tenn. :
|b distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy,
|c 1991.
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| 300 |
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|a 19 p. :
|b digital, PDF file.
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| 336 |
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|a text
|b txt
|2 rdacontent.
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| 337 |
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|a computer
|b c
|2 rdamedia.
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| 338 |
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|a online resource
|b cr
|2 rdacarrier.
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|a Published through the Information Bridge: DOE Scientific and Technical Information.
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| 500 |
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|a 12/31/1991.
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|a "doe/er/13546--4"
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|a "DE92018530"
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|a Interim;
|b 01/01/1989 - 12/31/1991.
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| 520 |
3 |
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|a In prior support periods we identified, cloned and sequenced three genes involved in the regulation of nif gene expression in Rhodobacter capsulatus. These were called nifRI, nifR2 and nifR4; they turn out to be homologue of the ntrC, ntrB and ntrA genes of enterobacteria. We subsequently found that mutations in an additional gene, nifR5. render R. capsulatus nif genes constitutive with respect to ammonia. The nifR5 gene was shown to be similar to glnB of enteric bacteria, encoding the regulatory protein PII, and furthering the intersection of the glutamine synthetase adenylylation cascade with the control of nif gene transcription. In pursuit of the mechanism of 0₂ control of nif gene expression, we constructed and analyzed the topology of a small plasmid in R. capsulatus as a function of 0₂ concentration. We also cloned and obtained partial sequence data for two genes encoding the B subunit of DNA gyrase. The nucleotide sequence of the rpoB gene encoding RNA polymerase was nearly completed. A method for isolation of genes expressed differentially, developed for cyanobacteria, was applied successfully to R. capsulatus. Several genes that depend on nifR4 for their transcription were isolated. A transcription start site for a nifA gene was identified and the promoter sequence was analyzed. A physical map of the R calsulatus SB1003 chromosome was prepared, based on pulsed-field electrophoresis of XbaI and AseI fragments and hybridization with a gridded cosmid library, using a device that permits 864 cosmids to be hybridized at one time with a labeled chromosomal fragment.
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| 536 |
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|b FG02-86ER13546.
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| 650 |
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7 |
|a Molecular Biology.
|2 local.
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| 650 |
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7 |
|a Gene Repressors.
|2 local.
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| 650 |
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|a Progress Report.
|2 local.
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| 650 |
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|a Gene Regulation.
|2 local.
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| 650 |
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|a Species Diversity.
|2 local.
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| 650 |
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|a Nitrogen Fixation.
|2 local.
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| 650 |
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7 |
|a Dna Sequencing.
|2 local.
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| 650 |
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7 |
|a Gene Operons.
|2 local.
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| 650 |
|
7 |
|a Electrophoresis.
|2 local.
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| 650 |
|
7 |
|a Rhodopseudomonas.
|2 local.
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| 650 |
|
7 |
|a Gene Mutations.
|2 local.
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| 650 |
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7 |
|a Dna Helicases.
|2 local.
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| 650 |
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7 |
|a Dna-cloning.
|2 local.
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| 650 |
|
7 |
|a Rna Polymerases.
|2 local.
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| 650 |
|
7 |
|a Genetic Mapping.
|2 local.
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| 650 |
|
7 |
|a Biomass Fuels.
|2 edbsc.
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| 710 |
2 |
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|a University of Chicago.
|4 res.
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| 710 |
1 |
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|a United States.
|b Department of Energy.
|4 spn.
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| 710 |
1 |
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|a United States.
|b Department of Energy.
|b Chicago Operations Office.
|4 res.
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| 710 |
1 |
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|a United States.
|b Department of Energy.
|b Office of Scientific and Technical Information.
|4 dst.
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| 856 |
4 |
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|u http://www.osti.gov/servlets/purl/10166528-5puDUB/
|z Online Access
|
| 907 |
|
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|a .b59825406
|b 03-06-23
|c 05-26-10
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| 998 |
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|a web
|b 05-26-10
|c f
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|p Can circulate
|a University of Colorado Boulder
|b Online
|c Online
|d Online
|e E 1.99:doe/er/13546--4
|h Superintendent of Documents classification
|i web
|n 1
|
