Biological (molecular and cellular) markers of toxicity. Final report, September 15, 1988--September 14, 1991 [electronic resource]
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| Online Access: |
Online Access |
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| Format: | Government Document Electronic eBook |
| Language: | English |
| Published: |
Washington, D.C. : Oak Ridge, Tenn. :
United States. Dept. of Defense ; distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy,
1991.
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| Abstract: | Several molecular and cellular markers of genotoxicity were adapted for measurement in the Medaka (Oryzias latipes), and were used to describe the effects of treatment of the organism with diethylnitrosamine (DEN). NO⁶-ethyl guanine adducts were detected, and a slight statistically significant, increase in DNA strand breaks was observed. These results are consistent with the hypothesis that prolonged exposure to high levels of DEN induced alkyltransferase activity which enzymatically removes any O⁶-ethyl guanine adducts but does not result in strand breaks or hypomethylation of the DNA such as might be expected from excision repair of chemically modified DNA. Following a five week continuous DEN exposure with 100 percent renewal of DEN-water every third day, the F values (DNA double strandedness) increased considerably and to similar extent in fish exposed to 25, 50, and 100 ppM DEN. This has been observed also in medaka exposed to BaP. |
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| Item Description: | Published through the Information Bridge: DOE Scientific and Technical Information. 12/15/1991. "ornl/m--1845" "DE92019190" Greeley, M.S. Jr.; Shugart, L.R.; Gettys-Hull, C.; D̀Surney, S.J. |
| Physical Description: | 86 p. : digital, PDF file. |
| Type of Report and Period Covered Note: | Topical; |