Vadose zone microbial community structure and activity in metal/radionuclide contaminated sediments. Final technical report [electronic resource]
Vadose Zone; Sediments; Chromium; Nitrates; Molasses; Microorganisms; Genetic Mapping; Clone Cells; Progress Report.
Saved in:
| Online Access: |
Online Access (via OSTI) |
|---|---|
| Corporate Author: | |
| Format: | Government Document Electronic eBook |
| Language: | English |
| Published: |
Washington, D.C. : Oak Ridge, Tenn. :
United States. Department of Energy. Office of Energy Research ; distributed by the Office of Scientific and Technical Information, U.S. Department of Energy,
2002.
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| Subjects: |
MARC
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| 245 | 0 | 0 | |a Vadose zone microbial community structure and activity in metal/radionuclide contaminated sediments. Final technical report |h [electronic resource] |
| 260 | |a Washington, D.C. : |b United States. Department of Energy. Office of Energy Research ; |a Oak Ridge, Tenn. : |b distributed by the Office of Scientific and Technical Information, U.S. Department of Energy, |c 2002. | ||
| 300 | |a 11 pages : |b digital, PDF file. | ||
| 336 | |a text |b txt |2 rdacontent. | ||
| 337 | |a computer |b c |2 rdamedia. | ||
| 338 | |a online resource |b cr |2 rdacarrier. | ||
| 500 | |a Published through SciTech Connect. | ||
| 500 | |a 08/17/2002. | ||
| 500 | |a "doe/er/62534-1" | ||
| 500 | |a Balkwill, David L. | ||
| 513 | |a Final; |b 09/01/2001 - 11/15/2001. | ||
| 520 | 3 | |a This final technical report describes the research carried out during the final two months of the no-cost extension ending 11/14/01. The primary goals of the project were (1) to determine the potential for transformation of Cr(VI) (oxidized, mobile) to Cr(III) (reduced, immobile) under unsaturated conditions as a function of different levels and combinations of (a) chromium, (b) nitrate (co-disposed with Cr), and (c) molasses (inexpensive bioremediation substrate), and (2) to determine population structure and activity in experimental treatments by characterization of the microbial community by signature biomarker analysis and by RT-PCR and terminal restriction fragment length polymorphism (T-RFLP) and 16S ribosomal RNA genes. It was determined early in the one-year no-cost extension period that the T-RFLP approach was problematic in regard to providing information on the identities of microorganisms in the samples examined. As a result, it could not provide the detailed information on microbial community structure that was needed to assess the effects of treatments with chromium, nitrate, and/or molasses. Therefore, we decided to obtain the desired information by amplifying (using TR-PCR, with the same primers used for T-RFLP) and cloning 16S rRNA gene sequences from the same RNA extracts that were used for T-RFLP analysis. We also decided to use a restriction enzyme digest procedure (fingerprinting procedure) to place the clones into types. The primary focus of the research carried out during this report period was twofold: (a) to complete the sequencing of the clones, and (b) to analyze the clone sequences phylogenetically in order to determine the relatedness of the bacteria detected in the samples to each other and to previously described genera and species. | |
| 520 | 0 | |a Vadose Zone; Sediments; Chromium; Nitrates; Molasses; Microorganisms; Genetic Mapping; Clone Cells; Progress Report. | |
| 536 | |b FG02-98ER62534. | ||
| 650 | 7 | |a Chromium. |2 local. | |
| 650 | 7 | |a Cloning. |2 local. | |
| 650 | 7 | |a Enzymes. |2 local. | |
| 650 | 7 | |a Genes. |2 local. | |
| 650 | 7 | |a Microorganisms. |2 local. | |
| 650 | 7 | |a Molasses. |2 local. | |
| 650 | 7 | |a Nitrates. |2 local. | |
| 650 | 7 | |a Ribosomal Rna. |2 local. | |
| 650 | 7 | |a Rna. |2 local. | |
| 650 | 7 | |a Sediments. |2 local. | |
| 650 | 7 | |a Genetic Mapping. |2 local. | |
| 650 | 7 | |a Clone Cells. |2 local. | |
| 650 | 7 | |a Progress Report. |2 local. | |
| 650 | 7 | |a Basic Biological Sciences. |2 edbsc. | |
| 650 | 7 | |a Environmental Sciences. |2 edbsc. | |
| 710 | 2 | |a Florida State University. |4 res. | |
| 710 | 1 | |a United States. |b Department of Energy. |b Office of Energy Research. |4 spn. | |
| 710 | 1 | |a United States. |b Department of Energy. |b Office of Scientific and Technical Information. |4 dst. | |
| 856 | 4 | 0 | |u http://www.osti.gov/scitech/biblio/807073 |z Online Access (via OSTI) |
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| 952 | f | f | |p Can circulate |a University of Colorado Boulder |b Online |c Online |d Online |e E 1.99:doe/er/62534-1 |h Superintendent of Documents classification |i web |n 1 |