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|a (TOE)ost894996
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|a (TOE)894996
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|a TOE
|c TOE
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|a GDWR
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|a 59
|2 edbsc
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|a E 1.99:conf-nabir2005-1
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|a E 1.99:conf-nabir2005-1
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|a conf-nabir2005-1
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|a Transcriptome and Proteome Dynamics of the Cellular Response of Shewanella oneidensis to Chromium Stress
|h [electronic resource]
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| 260 |
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|a Washington, D.C. :
|b United States. Dept. of Energy. Office of Science ;
|a Oak Ridge, Tenn. :
|b distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy,
|c 2005.
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| 336 |
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|a text
|b txt
|2 rdacontent.
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| 337 |
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|a computer
|b c
|2 rdamedia.
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| 338 |
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|a online resource
|b cr
|2 rdacarrier.
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| 500 |
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|a Published through the Information Bridge: DOE Scientific and Technical Information.
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|a 04/18/2005.
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|a "conf-nabir2005-1"
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|a Annual NABIR PI Meeting, April 18, 2005, Warrenton, VA.
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|a Thompson, D.K.
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|a University of Central Florida, Orlando, FL.
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|a The overall goal of this DOE NABIR project is to characterize the molecular basis and regulation of hexavalent chromium [Cr(VI)] stress response and reduction by Shewanella oneidensis strain MR-1. Temporal genomic profiling and mass spectrometry-based proteomic analysis were employed to characterize the dynamic molecular response of S. oneidensis MR-1 to both acute and chronic Cr(VI) exposure. The acute stress response of aerobic, mid-exponential phase cells shocked to a final concentration of 1 mM potassium chromate (K2CrO4) was examined at post-exposure time intervals of 5, 30, 60, and 90 min relative to untreated cells. The transcriptome of mid-exponential cultures was also analyzed 30 min after shock doses of 0.3, 0.5, or 1 mM K₂CrO₄. The tonB1-exbB1-exbD1 genes comprising the TonB1 iron transport system were some of the most highly induced coding sequences (CDSs) after 90 min (up to ≈240 fold), followed by other genes involved in heme transport, sulfate transport, and sulfur assimilation pathways. In addition, transcript levels for CDSs with annotated functions in DNA repair (dinP, recX, recA, recN) and detoxification processes (so3585, so3586) were substantially increased in Cr(VI)-exposed cells compared to untreated cells. By contrast, genes predicted to encode hydrogenases (HydA, HydB), oxidoreductases (SO0902-03-04, SO1911), iron-sulfur cluster binding proteins (SO4404), decaheme cytochrome c proteins (MtrA, OmcA, OmcB), and a number of LysR or TetR family transcriptional regulators were some of the most highly repressed CDSs following the 90-min shock period. Transcriptome profiles generated from MR-1 cells adapted to 0.3 mM Cr(VI) differed significantly from those characterizing cells exposed to acute Cr(VI) stress without adaptation. Parallel proteomic characterization of soluble protein and membrane protein fractions extracted from Cr(VI)-shocked and Cr(VI)-adapted MR-1 cells was performed using multidimensional HPLC-ESI-MS/MS (both LCQ and LTQ instruments used). With LTQ, we were able to substantially increase proteome coverage by at least two-fold compared to LCQ analysis. These studies provide important insights into cellular chromium tolerance. Future research will focus on the structural and regulatory genes implicated in Cr(VI) reduction and detoxification.
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|a Genes.
|2 local.
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|a Chromium.
|2 local.
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| 650 |
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|a Detoxification.
|2 local.
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|a Potassium.
|2 local.
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| 650 |
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|a Oxidoreductases.
|2 local.
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|a Sulfates.
|2 local.
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| 650 |
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|a Strains.
|2 local.
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|a Heme.
|2 local.
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| 650 |
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|a Chromates.
|2 local.
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| 650 |
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|a Cytochromes.
|2 local.
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| 650 |
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|a Membrane Proteins.
|2 local.
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| 650 |
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|a Proteins.
|2 local.
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| 650 |
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|a Dna Repair.
|2 local.
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| 650 |
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|a Sulfur.
|2 local.
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| 650 |
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7 |
|a Hydrogenases.
|2 local.
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| 650 |
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7 |
|a Iron.
|2 local.
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| 650 |
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|a Environmental Sciences.
|2 edbsc.
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| 650 |
|
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|a Basic Biological Sciences.
|2 edbsc.
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| 710 |
2 |
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|a Oak Ridge National Laboratory.
|4 res.
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| 710 |
1 |
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|a United States.
|b Department of Energy.
|b Office of Science.
|4 spn.
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| 710 |
1 |
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|a United States.
|b Department of Energy.
|b Environmental Management Science Program.
|4 res.
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| 710 |
1 |
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|a United States.
|b Department of Energy.
|b Office of Scientific and Technical Information.
|4 dst.
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| 856 |
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|u http://www.osti.gov/servlets/purl/894996-2NDu82/
|z Online Access
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| 907 |
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|a .b70246865
|b 03-06-23
|c 03-31-12
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| 998 |
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|a web
|b 03-31-12
|c f
|d m
|e p
|f eng
|g dcu
|h 0
|i 1
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| 956 |
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|a Information bridge
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|i 473fb87a-e46e-5081-b1c8-ca860ce30a4d
|s 0b0633bb-c230-50dc-bdb1-b7038a2de5f9
|
| 952 |
f |
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|p Can circulate
|a University of Colorado Boulder
|b Online
|c Online
|d Online
|e E 1.99:conf-nabir2005-1
|h Superintendent of Documents classification
|i web
|n 1
|
