Quantitive DNA Fiber Mapping [electronic resource]
Physical Mapping Dna Molecules Dna Fibers Fluorescence In Situ Hybridization Fish Digital Image Analysis.
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| Online Access: |
Online Access |
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| Format: | Government Document Electronic eBook |
| Language: | English |
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Berkeley, Calif. : Oak Ridge, Tenn. :
Lawrence Berkeley National Laboratory ; distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy,
2008.
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| Summary: | Physical Mapping Dna Molecules Dna Fibers Fluorescence In Situ Hybridization Fish Digital Image Analysis. |
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| Abstract: | Several hybridization-based methods used to delineate single copy or repeated DNA sequences in larger genomic intervals take advantage of the increased resolution and sensitivity of free chromatin, i.e., chromatin released from interphase cell nuclei. Quantitative DNA fiber mapping (QDFM) differs from the majority of these methods in that it applies FISH to purified, clonal DNA molecules which have been bound with at least one end to a solid substrate. The DNA molecules are then stretched by the action of a receding meniscus at the water-air interface resulting in DNA molecules stretched homogeneously to about 2.3 kb/μm. When non-isotopically, multicolor-labeled probes are hybridized to these stretched DNA fibers, their respective binding sites are visualized in the fluorescence microscope, their relative distance can be measured and converted into kilobase pairs (kb). The QDFM technique has found useful applications ranging from the detection and delineation of deletions or overlap between linked clones to the construction of high-resolution physical maps to studies of stalled DNA replication and transcription. |
| Item Description: | Published through the Information Bridge: DOE Scientific and Technical Information. 01/28/2008. "lbnl-120e" Wang, Mei; Weier, Heinz-Ulli G.; Weier, Jingly F.; Lu, Chun-Mei; Greulich-Bode, Karin M. Life Sciences Division. |