Digital microscopy / edited by Greenfield Sluder, David E. Wolf.

This book provides the essential tools and methods for working with digital microscopy as a technique for quantitative biological research.

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Bibliographic Details
Online Access: Full Text (via ProQuest)
Other Authors: Sluder, Greenfield, Wolf, D. E. (David E.)
Format: eBook
Language:English
Published: San Diego, Calif. : Academic Press, ©2013.
Edition:4th ed.
Series:Methods in cell biology ; v. 114.
Subjects:
Table of Contents:
  • Microscope basics / Greenfield Sluder, Joshua J. Nordberg
  • The optics of microscopic image foundation / David E. Wolf
  • Proper alignment of the microscope / Rudi Rottenfusser
  • Fundamentals of fluorescence and fluorescence microscopy / David E. Wolf
  • Fluorescent protein applications in microscopy / Whitney L. Johnson, Aaron F. Straight
  • Live-cell fluorescence imaging / Jennifer C. Waters
  • Practical aspects of adjusting digital cameras / Joshua J. Nordberg, Greenfield Sluder
  • Cameras for digital microscopy / Kenneth R. Spring
  • A high-resolution multimode digital microscope system / Edward D. Salmon, Sidney L. Shaw, Jennifer C. Waters, Clare M. Waterman-Storer, Paul S. Maddox, Elaine Yeh, Kerry Bloom
  • Electronic cameras for low-light microscopy / Ivan Rasnik, Todd French, Ken Jacobson, Keith Berland
  • Camera technologies for low light imaging : overview and relative advantages / Butch Moomaw
  • Post-processing for statistical image analysis in light microscopy / Richard A. Cardullo, Edward H. Hinchcliffe
  • 65,000 shades of grey : use of digital image files in light microscopy / Sidney L. Shaw, Edward H. Hinchcliffe
  • Quantitative analysis of digital microscope images / David E. Wolf, Champika Samarasekera, Jason R. Swedlow
  • Evaluating optical aberrations using fluorescent microspheres : methods, analysis, and corrective actions / Paul C. Goodwin
  • Ratio imaging : practical considerations in measuring intracellular CA2+ and pH in living cells / Nathan O'Connor, Randi B. Silver
  • Quantitative fluorescence microscopy and image deconvolution / Jason R. Swedlow
  • Practical aspects of quantitative confocal microscopy / John M. Murray
  • Theoretical principles and practical considerations for fluorescence resonance energy transfer microscopy / Richard A. Cardullo
  • Fluorescence lifetime imaging microscopy for quantitative biological imaging / Leng-Chun Chen, William R. Lloyd, III, Ching-Wei Chang, Dhruv Sud, Mary-Ann Mycek
  • Fluorescence correlation spectroscopy : molecular complexing in solution and in living cells / Dylan A. Bulseco, David E. Wolf
  • Breaking the resolution limit in light microscopy / Rainer Heintzmann, Gabriella Ficz
  • Circumventing photodamage in live-cell microscopy / Valentin Magidson, Alexey Khodjakov
  • A user's guide to localization-based super-resolution fluorescence imaging / Graham T. Dempsey
  • Quantitative ratiometric imaging of FRET-biosensors in living cells / Desiree Spiering, Jose Javier Bravo-Cordero, Yasmin Moshfegh, Veronika Miskolci, Louis Hodgson
  • Tip-enhanced raman spectroscopy for the base interrogation of DNA / Noah Kolodziejski.