In vitro mutagenesis protocols / edited by Jeff Braman.
In the post-genome era, in vitro mutagenesis has emerged as the critically important tool used by molecular biologists in establishing the functions of components of the proteome. In this second edition of In Vitro Mutagenesis Protocols, active researchers with proven track records describe in stepw...
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| Online Access: |
Full Text (via EBSCO) |
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| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press,
©2002.
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| Edition: | 2nd ed. |
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 182. |
| Subjects: |
| Summary: | In the post-genome era, in vitro mutagenesis has emerged as the critically important tool used by molecular biologists in establishing the functions of components of the proteome. In this second edition of In Vitro Mutagenesis Protocols, active researchers with proven track records describe in stepwise fashion their advanced mutagenesis techniques. Each contributor focuses on improvements to conventional site-directed mutagenesis, with chapters being devoted to chemical site-directed mutagenesis; PCR-based mutagenesis and the modifications that allow high-throughput experiments; and mutagenesis based on gene disruption that is both in vitro- and in situ-based. Additional methods are provided for in vitro gene evolution; for gene disruption based on transposon, recombination, and cassette mutagenesis; and for facilitating the introduction of multiple mutations. Each readily reproducible technique includes detailed step-by-step instructions, tips on pitfalls to avoid, and notes on reagents and suppliers. Time-tested and highly practical, the techniques in In Vitro Mutagenesis Protocols, Second Edition offer today's molecular biologists a rich compendium of reliable and powerful techniques with which to illuminate the proteome. |
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| Physical Description: | 1 online resource (xvi, 287 pages) : illustrations. |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: | 9781592591947 1592591949 0585403023 9780585403021 1280835915 9781280835919 9786610835911 6610835918 161737248X 9781617372483 9781489938947 148993894X |
| Language: | English. |
| Source of Description, Etc. Note: | Print version record. |