Biomass, community structure and nutritional status attributes of the deep subsurface microbiota---at Idaho and Hanford sites [electronic resource]

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Bibliographic Details
Online Access: Online Access
Main Authors: White, D.C (Author), Ringelberg, D.B (Author)
Corporate Author: University of Tennessee, Knoxville (Researcher)
Format: Government Document Electronic eBook
Language:English
Published: Washington, D.C. : Oak Ridge, Tenn. : United States. Department of Energy ; distributed by the Office of Scientific and Technical Information, U.S. Department of Energy, 1991.
Subjects:

MARC

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245 0 0 |a Biomass, community structure and nutritional status attributes of the deep subsurface microbiota---at Idaho and Hanford sites  |h [electronic resource] 
260 |a Washington, D.C. :  |b United States. Department of Energy ;  |a Oak Ridge, Tenn. :  |b distributed by the Office of Scientific and Technical Information, U.S. Department of Energy,  |c 1991. 
300 |a Pages: (18 p) :  |b digital, PDF file. 
336 |a text  |b txt  |2 rdacontent. 
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500 |a Published through SciTech Connect. 
500 |a 10/28/1991. 
500 |a "doe/er/60988-t1" 
500 |a "DE93003594" 
500 |a White, D.C.; Ringelberg, D.B. 
520 3 |a The signature lipid biomarker technique based on phospholipid ester-linked fatty acid pattern analysis (PLFA) provides data on the total viable or potentially viable communities without the necessity of: (1) Quantitative recovery from the sediments or (2) The ability to culture the organisms. Analysis of PLFA provides evidence for the nutritional status (starvation and/or unbalanced growth) in situ. PLFA analysis of SSP samples from the INEL and PNL sites vadose zones showed higher biomass at the surface with prominent Actinomyces biomarkers with lower biomasses of stressed microbiota at progressively greater depth. The biomass and community diversity increased at the water table at both sites. Both these Western sites showed lower viable microbial biomasses than the WSRS samples. Cluster analysis of the total patterns from various sedimentary horizons showed three major consortia of microbes, with surface microbiota related at both sites, low viable biomass sites closely related at both sites, with anaerobic desaturase pathway being predominant at INEL and consortia utilizing predominantly branched saturated and the aerobic desaturase pathway at both sites. Preliminary examination of the consortia recovered from NTS show a clear relationship to water level. 
536 |b FG05-90ER60988. 
650 7 |a Underground.  |2 local. 
650 7 |a Baseline Ecology.  |2 local. 
650 7 |a Bacteria.  |2 local. 
650 7 |a Biodegradation.  |2 local. 
650 7 |a Lipids.  |2 local. 
650 7 |a Micrococcus.  |2 local. 
650 7 |a Organic Wastes.  |2 local. 
650 7 |a Planning.  |2 local. 
650 7 |a Chemical Reactions.  |2 local. 
650 7 |a Decomposition.  |2 local. 
650 7 |a Ecology.  |2 local. 
650 7 |a Levels.  |2 local. 
650 7 |a Microorganisms.  |2 local. 
650 7 |a Organic Compounds.  |2 local. 
650 7 |a Wastes.  |2 local. 
650 7 |a Environmental Sciences.  |2 edbsc. 
650 7 |a Nuclear Fuel Cycle And Fuel Materials.  |2 edbsc. 
700 1 |a White, D.C.  |4 aut. 
700 1 |a Ringelberg, D.B.  |4 aut. 
710 2 |a University of Tennessee, Knoxville.  |4 res. 
710 1 |a United States.  |b Department of Energy.  |4 spn. 
710 1 |a United States.  |b Department of Defense.  |4 spn. 
710 2 |a Electric Power Research Institute.  |4 spn. 
710 1 |a United States.  |b National Aeronautics and Space Administration.  |4 spn. 
710 2 |a National Science Foundation (U.S.).  |4 spn. 
710 1 |a United States.  |b Department of Energy.  |b Office of Scientific and Technical Information.  |4 dst. 
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