Genetic transformation systems in fungi. Volume 1 / Marco A. van den Berg, Karunakaran Maruthachalam, editors.
Several different transformation techniques have been developed over the years and readily shown to be decisive methods in fungal biotechnology. This book will cover the basics behind the most commonly used transformation methods, as well as associated tools and techniques. Each chapter will provide...
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| Format: | eBook |
| Language: | English |
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[2014]
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| Series: | Fungal biology (Springer (Firm))
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| 245 | 0 | 0 | |a Genetic transformation systems in fungi. |n Volume 1 / |c Marco A. van den Berg, Karunakaran Maruthachalam, editors. |
| 264 | 1 | |a Cham : |b Springer, |c [2014] | |
| 264 | 4 | |c ©2015. | |
| 300 | |a 1 online resource (xv, 318 pages) : |b illustrations (some color) | ||
| 336 | |a text |b txt |2 rdacontent. | ||
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| 490 | 1 | |a Fungal biology, |x 2198-7777. | |
| 500 | |a Includes index. | ||
| 505 | 0 | |a Preface; Contents; Contributors; Part I: Introduction; 1: Fungal Transformation: From Protoplasts to Targeted Recombination Systems; 1.1 Introduction; 1.1.1 A Historical Perspective of Transformation of Fungi; 1.2 Early Development of Basic Tools for Transformation; 1.2.1 Protoplasts and Lytic Enzymes; 1.2.2 Osmotic Stabilization and Protoplasts Regeneration; 1.3 Alternative Transformation Procedures That Do Not Require Protoplasts; 1.3.1 Electroporation; 1.3.2 Agrobacterium-Mediated Transformation; 1.3.3 Biolistic Transformation; 1.4 Selective Markers; 1.4.1 Nutritional Markers. | |
| 505 | 8 | |a 1.4.2 Metabolic Fitness of Auxotrophic Host Strains: Growth and Secondary Metabolites Production1.4.3 Positive Selection Resistance Markers; 1.5 Autonomously Replicating Plasmids: Stability Problems of Gene Libraries; 1.6 Homologous and Nonhomologous Recombination: Integration of Exogenous Genes; 1.6.1 Targeted Monocopy Integrations at Specific Loci; 1.6.2 The "Two Markers" Selection Strategy for Gene Disruption; 1.6.3 The Nonhomologous End-Joining Mechanism; 1.6.4 Metabolic Fitness of NHEJ-ƯDeficient Mutants; 1.7 Future Outlook; References. | |
| 505 | 8 | |6 880-01 |a 2.3.1.1 Cell Type and Growth Phase2.3.1.2 Growth Conditions; 2.3.1.3 Optimal Enzyme Combination for Lysing the Cell Wall; 2.3.1.4 Stabilizing Protoplasts During and After Removal of the Cell Wall; 2.3.1.5 Incubation Time and Temperature for Lysing the Cell Wall; 2.3.1.6 Evaluation and Storage of Protoplasts; 2.3.1.7 Uptake of DNA/Transformation; 2.3.1.8 Incubation Time and Medium for Protoplast Regeneration; 2.4 Concluding Remarks; References; 3: Trichoderma Transformation Methods; 3.1 General Methods; 3.2 Detailed Procedure Description. | |
| 505 | 8 | |a 3.2.1 Transformation of Trichoderma Mediated by Protoplasts3.2.1.1 Growth Conditions and Protoplasts Formation; 3.2.1.2 Protoplasts Transformation; 3.2.2 Transformation of Trichoderma Mediated by A. tumefaciens; 3.3 Alternative Trichoderma Transformation Procedures; 3.3.1 Protoplast Electroporation (S©Łnchez-Torres et al. 1994); 3.3.2 Biolistic (Lorito et al. 1993); 3.3.3 Shock Waves (Maga©ła-Ort©Ưz et al. 2013); 3.4 Notes; References; 4: Transformation of Mucor circinelloides f. lusitanicus Protoplasts; 4.1 Introduction; 4.2 Materials; 4.3 Methods; 4.3.1 M. circinelloides Cell-Wall Isolation. | |
| 520 | |a Several different transformation techniques have been developed over the years and readily shown to be decisive methods in fungal biotechnology. This book will cover the basics behind the most commonly used transformation methods, as well as associated tools and techniques. Each chapter will provide protocols along with examples used in laboratories worldwide. Not only will this text provide a detailed background on applications in industrial and pharmaceutical relevant microbes, but also the importance of fungal pathogens in agricultural production (Phytophthora and Botrytis) and mammalia. | ||
| 588 | 0 | |a Online resource; title from PDF title page (SpringerLink, viewed November 21, 2014) | |
| 650 | 0 | |a Fungi |x Genetics. |0 http://id.loc.gov/authorities/subjects/sh85052445. | |
| 650 | 7 | |a Fungi |x Genetics. |2 fast |0 (OCoLC)fst00936316. | |
| 700 | 1 | |a Berg, Marco A. van den, |e editor. | |
| 700 | 1 | |a Maruthachalam, Karunakaran, |e editor. | |
| 776 | 0 | 8 | |i Print version: |a Van den Berg, Marco A. |t Genetic Transformation Systems in Fungi, Volume 1. |d Cham : Springer International Publishing, ©2014 |z 9783319101415. |
| 830 | 0 | |a Fungal biology (Springer (Firm)) |0 http://id.loc.gov/authorities/names/no2013039118. | |
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| 880 | 8 | |6 505-01/(S |a Part II: Transformation Methods: Protoplast Transformation2: Protoplast Transformation for Genome Manipulation in Fungi; 2.1 Introduction; 2.2 The Fungal Cell Wall: A Barrier to be Removed; 2.2.1 Composition of the Fungal Cell Wall; 2.2.2 Functions and Biological Activity of the Fungal Cell Wall; 2.2.2.1 Chitin; 2.2.2.2 Glucans; 2.2.2.3 Non-cellulosic β-Glucans; 2.2.2.4 Mannans; 2.2.2.5 Proteins; 2.2.3 Enzymes, Biosynthesis, and Degradation of the Fungal Cell Wall; 2.3 Getting Foreign DNA into the Fungal Cell; 2.3.1 Removing the Cell Wall: Protoplast Preparation. | |
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