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A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis / edited by Thomas Meier, Falk Fahrenholz.

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Bibliographic Details
Online Access: Full Text (via Springer)
Main Author: Meier, Thomas
Other Authors: Fahrenholz, F. (Falk)
Format: eBook
Language:English
Published: Basel : Birkhäuser Basel, 1995.
Series:BioMethods ; v. 7.
Subjects:
Life sciences.
Medicine.
Table of Contents:
  • 1 An Introduction to Avidin-Biotin Technology and Options for Biotinylation
  • 1.1 Introduction
  • 1.2 Technical Procedures
  • 1.3 Troubleshooting
  • Acknowledgments
  • References
  • 2 Synthesis of Photocleavable Biotinylated Ligands and Application for Affinity Chromatography
  • Summary
  • 2.1 Introduction
  • 2.2 Technical Procedures
  • 2.3 Results and Discussion
  • 2.4 Troubleshooting
  • References
  • 3 Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands
  • Summary
  • 3.1 Introduction
  • 3.2 Technical Procedures
  • 3.3 Results and Discussion
  • 3.4 Troubleshooting
  • Acknowledgments
  • References
  • 4 Photoreactive Biotinylated Peptide Ligands for Affinity Labeling
  • Summary
  • 4.1 Introduction
  • 4.2 Technical Procedures
  • 4.3 Results and Discussion
  • 4.4 Troubleshooting
  • References
  • 5 Immunoprecipitation of Biotinylated Cell Surface Proteins
  • Summary
  • 5.1 Introduction
  • 5.2 Technical Procedures
  • 5.3 Results and Discussion
  • 5.4 Troubleshooting
  • Acknowledgments
  • References
  • 6 Biotinylation and Chemical Cross-Linking of Membrane Associated Molecules
  • Summary
  • 6.1 Introduction
  • 6.2 Technical Procedures
  • 6.3 Results and Discussion
  • 6.4 Troubleshooting
  • Acknowledgments
  • References
  • 7 Preparation of Biotinylated Lectins and Application in Microtiter Plate Assays and Western Blotting
  • Summary
  • 7.1 Introduction
  • 7.2 Technical Procedures
  • 7.3 Results and Discussion
  • 7.4 Troubleshooting
  • Acknowledgements
  • References
  • 8 Biotin-Labeling of Poly(ADP-ribose) in Poly(ADP-ribose)-Protein Interactions
  • Summary
  • 8.1 Introduction
  • 8.2 Technical Procedures
  • 8.3 Results and Discussion
  • 8.4 Troubleshooting
  • Acknowledgments
  • References
  • 9 Preparation and Use of Biotinylated Probes for the Detection and Characterisation of Serine Proteinase and Serine Proteinas Inhibitory Proteins
  • Summary
  • 9.1 Introduction
  • 9.2 Technical Procedures
  • 9.3 Results and Discussion
  • 9.4 Troubleshooting
  • Acknowledgments
  • References
  • 10 Avidin/Biotin-Mediated Conjugation of Antibodies to Erythrocytes: An Approach for Immunoerythrocyte Exploration in vivo
  • Summary
  • 10.1 Introduction
  • 10.2 Technical Procedures
  • 10.3 Results and Discussion
  • 10.4 Troubleshooting
  • Acknowledgments
  • References
  • 11 Biotin in vitro Translation: A Nonradioactive Method for the Synthesis of Biotin-Labeled Proteins in a Cell-Free System
  • Summary
  • 11.1 Introduction
  • 11.2 Technical Procedures
  • 11.3 Results and Discussion
  • 11.4 Troubleshooting
  • Acknowledgments
  • References
  • 12 Nonradioactive Detection of Nucleic Acids with Biotinylated Probes
  • Summary
  • 12.1 Introduction
  • 12.2 Technical procedures
  • 12.3 Results and discussion
  • 12.4 Troubleshooting
  • References
  • 13 Biotin-Labeled Riboprobes to Study RNA-Binding Proteins
  • Summary
  • 13.1 Introduction
  • 13.2 Technical Procedures
  • 13.3 Results and Discussion
  • 13.4 Troubleshooting
  • Acknowledgments
  • References
  • 14 Rapid YAC End Sequencing by Alu-Vector PCR and Biotinylated Primers
  • Summary
  • 14.1 Introduction
  • 14.2 Technical Procedures
  • 14.3 Results and Discussion
  • 14.4 Troubleshooting
  • Acknowledgments
  • References.

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