Functional Neuroanatomy / edited by Nicholas J. Strausfeld.
Saved in:
| Online Access: |
Full Text (via Springer) |
|---|---|
| Main Author: | |
| Format: | eBook |
| Language: | English |
| Published: |
Berlin, Heidelberg :
Springer Berlin Heidelberg,
1983.
|
| Series: | Springer series in experimental entomology.
|
| Subjects: |
Table of Contents:
- 1 Electron Microscopy of Golgi-Impregnated Neurons
- I. Introduction
- II. General Description of the Procedure
- III. Results
- IV. Discussion
- V. Appendix: Schedules
- 2 Block Intensification and X-Ray Microanalysis of Cobalt-Filled Neurons for Electron Microscopy
- I. Introduction
- II. Method
- III. Ultrastructure
- IV. Identification of Size and Nature of Precipitate
- V. Discussion
- VI. Applications
- 3 Horseradish Peroxidase and Other Heine Proteins as Neuronal Markers
- I. Introduction
- II. The Versatility of Exogenous Heme Proteins as Neuronal Markers
- III. Chemistry of Peroxidase-Active Proteins and Reactions for their Demonstration
- IV. Methods
- V. Cytology of Peroxidase-Labelled Neurons
- VI. Mechanisms of Neuronal Uptake and Transport of Herne Proteins
- VII. Concluding Remarks
- VIII. Addendum
- IX. Appendix 1: Method
- X. Appendix 2: Solutions
- 4 Intracellular Staining with Nickel Chloride
- I. Introduction
- II. Method
- 5 Rubeanic Acid and X-Ray Microanalysis for Demonstrating Metal Ions in Filled Neurons
- I. Introduction
- II. Use of Different Metal Ions
- III. Rubeanic Acid Development
- IV. Applications of Rubeanic Acid Development
- V. X-Ray Microanalysis for Detection of Metal Ions
- VI. Conclusion
- 6 Double Marking for Light and Electron Microscopy
- I. Introduction
- II. Double Marking for Light Microscopy
- III. Double Marking for Light and Electron Microscopy
- IV. Alternative Strategies
- 7 Lucifer Yellow Histology
- I. Introduction
- II. Filling from Electrodes
- III. Passive Back- or Forwardfilling
- IV. Fixing
- V. Buffers, Ringers
- VI. Whole-Mount Viewing
- VII. Embedding and Sectioning
- VIII. Microscopy
- IX. Photography
- X. Fading
- XI. Reconstructions
- XII. Geography
- XIII. Storage
- XIV. Artefacts
- XV. Conclusions
- 8 Portraying the Third Dimension in Neuroanatomy
- I. Introduction
- II. Why Computer Graphics in Neuroanatomy?
- III. Designing the System
- IV. The NEU System
- V. Alignment of Sections
- VI. Interactive Profile Acquisition
- VII. Noninteractive Operations
- VIII. Final Remarks
- 9 Three-Dimensional Reconstruction and Stereoscopic Display of Neurons in the Fly Visual System
- I. Introduction
- II. Procedure
- III. Hardware Configuration
- IV. The Data Acquisition Program HISDIG
- V. The Reproduction Program HISTRA
- VI. Stereoscopic Vision
- VII. Examples of Displays and Stereopairs
- VIII. Further Applications
- IX. Concluding Remarks
- 10 Laser Microsurgery for the Study of Behaviour and Neural Development of Flies
- I. Introduction
- II. The Laser Microbeam Unit
- III. Procedure of Laser Surgery
- IV. Histological Analysis
- V. Anatomical-Behavioural Correlations of Laser-Eliminated Lobula Plate Neurons
- VI. Aspects of Neuronal Development
- VII. Discussion
- 11 Anatomical Localization of Functional Activity in Flies Using 3H-2-Deoxy-D-Glucose
- I. Introduction
- II. Essentials of the Technique
- III. Results
- IV. Concluding Remarks
- 12 Strategies for the Identification of Amine- and Peptide-Containing Neurons
- I. Introduction
- II. Neutral Red: A Nonspecific Stain for Amine-Containing Neurons
- III. Neutral Red: An Indicator of Peptidergic Neurons
- IV. Permanent Preparations of Vital Staining with Neutral Red
- V. Use of Immunohistochemical Approaches to Neuron Identification
- VI. Immunohistochemical Screening: Whole-Mount Method
- VII. Identification: Immunohistochemistry and Dye Injection
- VIII. Confirmation of Immunohistochemistry: Cell Isolation, Extraction and Assay
- IX. Concluding Remarks
- 13 Immunochemical Identification of Vertebrate-Type Brain-Gut Peptides in Insect Nerve Cells
- I. Introduction
- II. Immunocytochemistry: Basic Principles
- III. Techniques of Immunocytochemistry
- IV. Problems of Specificity
- V. Brain-Gut Peptides in Insects
- VI. Extraction and Purification
- VII. Conclusions
- VIII. Appendix 1: Immunofluorescence: The Indirect Method
- IX. Appendix 2: Immunoperoxidase: The PAP Method
- 14 Immunocytochemical Techniques for the Identification of Peptidergic Neurons
- I. Introduction and Survey
- H. Preparation of Antigens
- III. Production and Isolation of Antibodies
- IV. Absorption of the Antisera Before Use for Immunocytochemistry
- V. Isolation of Hapten-Specific Antibodies
- VI. Methods for Antibody Isolation
- VII. Immunocytochemical Techniques
- VIII. Immunocytochemical Staining Methods
- IX. CoCl2 Iontophoresis and Indirect Immunofluorescence Method
- X. Supplementary Methods
- XI. Electron Microscopy
- XII. Conclusions
- 15 Detection of Serotonin-Containing Neurons in the Insect Nervous System by Antibodies to 5-HT
- I. Introduction
- II. General Considerations of Antibody Staining
- III The Immunofluorescence Technique
- IV. Fluorescence Microscopy and Photography
- V. The Unlabelled Antibody Enzyme Method for Sections
- VI. A Whole-Mount Method for Antibody Staining
- VII. Specificity of Anti-5-HT Labelling
- 16 Monoaminergic Innervation in a Hemipteran Nervous System: A Whole-Mount Histofluorescence Survey
- I. Introduction
- II. Materials and Methods
- III. Results
- IV. Discussion
- 17 Identification of Neurons Containing Vertebrate-Type Brain-Gut Peptides by Antibody and Cobalt Labelling
- I. Introduction
- II. Method
- III. Interpretation of the Results
- IV. Conclusions
- 18 Interpretation of Freeze-Fracture Replicas of Insect Nervous Tissue
- I. Introduction
- II. Procedure
- III. Interpretation of Replicas
- IV. The Cleaved Cell: A Survey
- V. Recent Advances and Future Prospects
- 19 High-Voltage Electron Microscopy for Insect Neuroanatomy
- I. Introduction
- II. Rationale for HVEM for Biological Research
- III. Method
- IV. HVEM of Insect Neurons
- References.