Osteoporosis [electronic resource] : methods and protocols / Jennifer J. Westendorf, editor.
In recent years, the study of bone cells and tissues at the cellular and molecular levels in a variety of models has revolutionized the field. In Osteoporosis: Methods and Protocols, leading scientists from around the world share their step-by-step laboratory protocols for studying bone biology. The...
Saved in:
| Online Access: |
Online Access |
|---|---|
| Other Authors: | |
| Format: | Electronic eBook |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
c2008.
|
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 455. |
| Subjects: |
Table of Contents:
- In vitro models. Isolation and culture of rodent osteoprogenitor cells / Thomas A. Owen and Lydia C. Pan. Osteoclast culture and resorption assays / Elizabeth W. Bradley and Merry Jo Oursler. Assessing new bone formation in neonatal calvarial organ cultures / Khalid S. Mohammad, John M. Chirgwin, and Theresa A. Guise. Detection of apoptosis of bone cells in vitro / Teresita Bellido and Lilian I. Plotkin
- In vivo rodent models. In vivo parathyroid hormone treatments and RNA isolation and analysis / Xin Li, Ling Qin, and Nicola C. Partridge. Assessment of bone formation capacity using in vivo transplantation assays : procedure and tissue analysis / Basem M. Abdallah, Nicholas Ditzel, and Moustapha Kassem. Ossicle and vossicle implant model systems / Glenda J. Pettway and Laurie K. McCauley. Utility of the ovariectomized rat as a model for human osteoporosis in drug discovery / Yogendra P. Kharode, Michael C. Sharp, and Peter V.N. Bodine. Orchidectomy models of osteoporosis / Stéphane Blouin ... [et al.]
- Methods of gene delivery. Gene delivery by adenoviruses / Renny T. Franceschi and Chunxi Ge. Lentivirus delivery of shRNA constructs into osteoblasts / Marc N. Wein, Dallas C. Jones, and Laurie H. Glimcher. Gene delivery by retroviruses / Valerie Deregowski and Ernesto Canalis
- Molecular and genetic techniques. Chromatin immunoprecipitation assays : application of ChIP-on-chip for defining dynamic transcriptional mechanisms in bone cells / Margaretha van der Deen ... [et al.] Identification of transcription factor target genes by ChIP display / Artem Barski, Steven Pregizer, and Baruch Frenkel. Application of the laser capture microdissection technique for molecular definition of skeletal cell differentiation in vivo / Dafna Benayahu, Rina Socher, and Irena Shur. Quantitative trait loci mapping / Dong-Hai Xiong ... [et al.]
- Imaging techniques. In situ nuclear organization of regulatory machinery / Shirwin M. Pockwinse ... [et al.] Bioluminescent imaging in bone / Yoram Zilberman ... [et al.] Micro-computed tomography : a method for the non-destructive evaluation of the three-dimensional structure of biological specimens / Martin Stauber and Ralph Müller. Fourier transform-infrared microspectrosopy and microscopic imaging / Samuel Gourion-Arsiquaud, Paul A. West, and Adele L. Boskey. Mechanical testing. Assessment of the in vivo adaptive response to mechanical loading / Leanne Kaye Saxon and Lance Edward Lanyon. Determination of bone's mechanical matrix properties by nanoindentation / Engin Ozcivici ... [et al.] Fluid flow assays / Ryan C. Riddle, Amanda F. Taylor and Henry J. Donahue.