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Human pluripotent stem cells [electronic resource] : methods and protocols / edited by Philip H. Schwartz, Robin L. Wesselschmidt.

Almost daily, new technologies are being presented that move the field of human pluripotent stem cell research towards a future that may yield highly-effective, personalized medical treatments. Three enabling technologies at hand for human PSCs are 1) directed reprogramming of somatic cells, which e...

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Bibliographic Details
Online Access: Online Access
Other Authors: Schwartz, Philip H., Wesselschmidt, Robin L.
Format: Electronic eBook
Language:English
Published: New York : Humana Press, c2011.
Series:Methods in molecular biology (Clifton, N.J.) ; v. 767.
Subjects:
Embryonic stem cells > Research.
Table of Contents:
  • The Stem Cell Laboratory: Design, Equipment, and Oversight
  • Stem Cell Banks: Preserving Cell Lines, Maintaining Genetic Integrity, and Advancing Research
  • Embryonic Stem Cell Derivation from Human Embryos
  • Derivation of Human Parthenogenetic Stem Cell Lines
  • Generation of Induced Pluripotent Stem Cell Lines from Human Fibroblasts via Retroviral Gene Transfer
  • Derivation of Induced Pluripotent Stem Cells by Lentiviral Transduction
  • Transgene-free Production of Pluripotent Stem Cells using PiggyBac Transposons
  • Traditional Human Embryonic Stem Cell Culture
  • Xeno-Free Culture of Human Pluripotent Stem Cells
  • Adaptation of Human Pluripotent Stem Cells to Feeder-Free Conditions in Chemically-Defined Medium with Enzymatic Single-Cell Passaging
  • GMP Scale-Up and Banking of Pluripotent Stem Cells for Cellular Therapy Applications
  • Culture of Human Pluripotent Stem Cells on Glass Slides for High-Resolution Imaging
  • Classical Cytogenetics: Karyotyping Techniques
  • FISH Analysis of Human Pluripotent Stem Cells
  • Immunocytochemical Analysis of Human Pluripotent Stem Cells
  • Flow Cytometric Analysis of Human Pluripotent Stem Cells
  • The Teratoma Assay: An In vivo Assessment of Pluripotency
  • Detection of Copy Number Variation using SNP Genotyping
  • Genome-wide Epigenetic Analysis of Human Pluripotent Stem Cells by ChIP and ChIP-Seq
  • Basic Approaches to Gene Expression Analysis of Stem Cells by Microarrays
  • Development of High Content Screening Approaches and Analysis for Human Pluripotent Stem Cells
  • Quantitative Proteome and Phosphoproteome Analysis of Human Pluripotent Stem Cells
  • Lentivirus-mediated Modification of Pluripotent Stem Cells
  • Nucleofection of Human Embryonic Stem Cells
  • Non-viral Gene Delivery in Neural Progenitors Derived from Human Pluripotent Stem Cells
  • Gene Targeting in Human Pluripotent Stem Cells
  • Episomal Transgene Expression in Pluripotent Stem Cells
  • The Generation of Embryoid Bodies from Feeder-based or Feeder-free Human Pluripotent Stem Cell Cultures
  • Derivation of Oligodendrocyte Progenitor Cells from Human Embryonic Stem Cells
  • Directed Differentiation of Dopamine Neurons From Human Pluripotent Stem Cells
  • Methods for the Derivation and Use of Cardiomyocytes from Human Pluripotent Stem Cells
  • In Vivo Evaluation of Putative Hematopoietic Stem Cells Derived from Human Pluripotent Stem Cells
  • Differentiation of Dendritic Cells from Human Embryonic Stem Cells.

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