Diabetes mellitus [electronic resource] : methods and protocols / edited by Sabire Özcan.
About 17 million Americans suffer from diabetes mellitus, a disease caused by defects in insulin production, insulin action, or both, and also a leading cause of kidney failure, blindness, and amputations. In Diabetes Mellitus: Methods and Protocols, leading researchers and clinical investigators de...
Saved in:
| Online Access: |
Full Text (via Springer) |
|---|---|
| Other Authors: | |
| Format: | Electronic eBook |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana,
2003.
|
| Series: | Methods in molecular medicine ;
83. |
| Subjects: |
MARC
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| 245 | 0 | 0 | |a Diabetes mellitus |h [electronic resource] : |b methods and protocols / |c edited by Sabire Özcan. |
| 260 | |a Totowa, N.J. : |b Humana, |c 2003. | ||
| 300 | |a 1 online resource (x, 192 pages) : |b illustrations. | ||
| 336 | |a text |b txt |2 rdacontent. | ||
| 337 | |a computer |b c |2 rdamedia. | ||
| 338 | |a online resource |b cr |2 rdacarrier. | ||
| 490 | 1 | |a Methods in molecular medicine ; |v 83. | |
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | |a Isolation of islets of Langerhans from rodent pancreas / C.B. Kelly [and others] -- Purification of rat pancreatic B-cells by fluorescence-activated cell sorting / G. Stange, M. Van De Casteele and H. Heimberg -- Assessment of insulin secretion in the mouse / M. Brissova [and others] -- Detection of insulin production by immunohistochemistry / S.C. Campbell and W.M. Macfarlane -- Quantification of the level of insulin gene expression / S.C. Campbell and W.M. Macfarlane -- Chromatin immunoprecipitation using isolated islets of Langerhans / M. Parrizas [and others] -- Adenoviral gene transfer into beta-cell lines / A.L. Mosley and S. Ozcan -- Utilization of NOD mice in the study of type 1 diabetes / D.G. Karounos and S.E. Goes -- Introduction of DNA into 3T3-L1 adipocytes by electroporation / S. Okada, M. Mori and J.E. Pessin -- Analysis of insulin-stimulated glucose uptake in differentiated 3T3-L1 adipocytes / J. Lakshmanan, J.S. Elmendorf and S. Ozcan -- Fractionation analysis of the subcellular distribution of GLUT-4 in 3T3-L1 adipocytes / J.S. Elmendorf -- Visualization and quantitation of integral membrane proteins using a plasma membrane sheet assay / J.B. Knight and A.L. Olson -- Assaying tyrosine phosphorylation of insulin receptor and insulin receptor substrates / E.L. Whiteman and M.J. Birnbaum -- Measuring insulin-stimulated phosphatidyl-inositol 3-kinase activity / L.P. Wang and S.A. Summers -- Assaying AKT/protein kinase B activity / K.L. Hoehn and S.A. Summers -- Measurements of cellular phosphoinositide levels in 3T3-L1 adipocytes / D.B. DeWald -- Measurement of glycogen synthesis and glycogen synthase activity in 3T3-L1 adipocytes / M.J. Brady -- Measurement of contraction-stimulated GLUT-4 translocation in isolated skeletal muscle / J.T. Brozinick, Jr. -- Single-embryo measurement of basal- and insulin-stimulated glucose uptake / M.M. Chi and K.H. Moley -- Immunohistologic staining of muscle and embryos to detect insulin-stimulated translocation of glucose transporters / M.O. Carayannopoulos and K.H. Moley. | |
| 520 | |a About 17 million Americans suffer from diabetes mellitus, a disease caused by defects in insulin production, insulin action, or both, and also a leading cause of kidney failure, blindness, and amputations. In Diabetes Mellitus: Methods and Protocols, leading researchers and clinical investigators describe their cutting-edge techniques for studying these defects at both the molecular and biochemical levels. Written in step-by-step detail to ensure ready reproducibility and robust results, these techniques allow investigators, both novices and those already active in the field, to study every major facet of insulin production and action. Each protocol includes an introduction to the technique, an explanation of its application, and a list of materials. Practical notes discuss how to avoid pitfalls, as well as how to adapt the methods to your own research. Cutting-edge and highly practical, Diabetes Mellitus: Methods and Protocols provides basic scientists and clinical researchers, both experienced investigators and those new to the field, all the critical tools needed for the productive study of diabetes mellitus today. | ||
| 588 | 0 | |a Print version record. | |
| 650 | 0 | |a Diabetes |x Molecular aspects |v Laboratory manuals. | |
| 650 | 7 | |a Diabetes |x Molecular aspects. |2 fast |0 (OCoLC)fst00892173. | |
| 655 | 7 | |a Laboratory manuals. |2 fast |0 (OCoLC)fst01920776. | |
| 700 | 1 | |a Özcan, Sabire. | |
| 776 | 0 | 8 | |i Print version: |t Diabetes mellitus. |d Totowa, N.J. : Humana, 2003 |z 1588291480 |z 9781588291486 |w (DLC) 2002032816 |w (OCoLC)50554899. |
| 830 | 0 | |a Methods in molecular medicine ; |v 83. | |
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