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Methods in Cell-Matrix Adhesion / edited by Josephine C. Adams ; prepared under the auspices of the American Society for Cell Biology.

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Critically acclaimed for more than 25 years, the Methods in Cell Biology series provides an indispensable tool for the researcher. Each volume is carefully edited by experts to contain state-of-the-art reviews and step-by-step protocols. Techniques are described completely so that methods are made a...

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Bibliographic Details
Online Access: Full Text (via ScienceDirect)
Other Authors: Adams, Josephine C. (Editor)
Format: eBook
Language:English
Published: San Diego : Academic Press, 2002.
Series:Methods in cell biology ; v. 69.
Subjects:
Cell adhesion.
Extracellular matrix.
Table of Contents:
  • pt. 1. Preface and perspectives on cell-matrix adhesion
  • Cell-matrix interactions: the view from the outside / Paul Bornstein
  • Matrix and meaning / Martin A. Schwartz
  • Cell-matrix adhesion research and the development of biotherapeutics / Roy R. Lobb
  • pt. 2. Matrix methodologies
  • Detection and purification of instructive extracellular matrix components with monoclonal antibody technologies / Susana G. Gil
  • Isolation and purification of proteoglycans / John M. Whitelock
  • Expression of recombinant matrix components using baculoviruses / Deane F. Mosher
  • Heparan sulfate
  • growth factor interactions / Alan C. Rapraeger
  • Analysis of basement membrane self-assembly and cellular interactions with native and recombinant glycoproteins / Peter D. Yurchenco
  • Preparation and analysis of synthetic multicomponent extracellular matrix / Kim S. Midwood
  • Analysis of matrix dynamics by atomic force microscopy / Helen G. Hansma
  • Analysis of matrix degradation / H.G. Munshi
  • pt. 3. Adhesion receptor methodologies
  • Expression cloning strategies for the identification of adhesion molecules / Joe W. Ramos
  • Purification of integrins and characterization of integrin-associated proteins / Johannes A. Eble
  • Methods for analysis of the integrin ligand binding event / Jeffrey W. Smith
  • Intracellular coupling of adhesion receptors: molecular proximity measurements / Maddy Parsons
  • pt. 4. Functional applications of cell-matrix adhesion in molecular cell biology
  • Functional analysis of cell adhesion: quantiation of cell-matrix attachment / Steven K. Akiyama
  • Measurements of glycosaminoglycan-based cell interactions / J. Kevin Langford
  • Applications of adhesion molecule gene knockout cell lines / Jordan A. Kreidberg
  • Flexible polyacrylamide substrata for the analysis of mechanical interactions at cell-substratum adhesions / Karen A. Beningo
  • Cell migration in slice cultures / Donna J. Webb
  • Application of cell adhesion to study signaling networks / Cindy K. Miranti
  • Use of micropatterned adhesive surfaces for control of cell behavior / Philip LeDuc
  • Adenoviral-mediated gene transfer in two-dimensional and three-dimensional cultures of mammary epithelial cells / Harriet Watkin.

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