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Retroviral proteases [electronic resource] / edited by Lawrence C. Kuo, Jules A. Shafer.

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Methods included in this volume apply to the expression and characterization of retroviral proteases and their inhibitor/substrate design.

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Bibliographic Details
Online Access: Full Text (via ScienceDirect)
Other Authors: Kuo, Lawrence C. (Editor), Shafer, Jules A. (Editor)
Format: Electronic eBook
Language:English
Published: San Diego : Academic Press, ©1994.
Series:Methods in enzymology ; v. 241.
Subjects:
Proteolytic enzymes.
Retroviruses > Enzymes.
HIV (Viruses)
Aspartic proteinases.
Table of Contents:
  • 1. Expression Systems for Retroviral Proteases / Jeffrey Stebbins and Christine Debouck
  • 2. Genetic Approaches Designed to Minimize Cytotoxicity of Retroviral Protease / C.J. Rizzo and B.D. Korant
  • 3. Host Strain Selection for Bacterial Expression of Toxic Proteins / Elizabeth Chen
  • 4. Assay Methods for Retroviral Proteases / Christopher U.T. Hellen
  • 5. Analysis of Human Immunodeficiency Virus Type 1 Protease Activity in Eukaryotic and Bacterial Cells / Andrew H. Kaplan, Marianne Manchester, Lorraine Everitt and Ronald Swanstrom
  • 6. Synthetic Approaches to Continuous Assays of Retroviral Proteases / Grant A. Krafft and Gary T. Wang
  • 7. Identification, Purification, and Cell Culture Assays of Retroviral Proteases / Klaus von der Helm, Sigrio Seelmeier, Alexei Kisselev and Hans Nitschko
  • 8. Stability of Dimeric Retroviral Proteases / Paul L. Darke.
  • 9. Use of Steady State Kinetic Methods to Elucidate the Kinetic and Chemical Mechanisms of Retroviral Proteases / Thomas D. Meek, Evelyn J. Rodriguez and Thelma S. Angeles
  • 10. X-Ray Structures of Retroviral Proteases and Their Inhibitor-Bound Complexes / Dagmar Ringe
  • 11. Probing Structure-Function Relationships in Human Immunodeficiency Virus Type 1 Protease via Molecular Dynamics Simulation / William E. Harte, Jr. and David L. Beveridge
  • 12. Relationships of Human Immunodeficiency Virus Protease with Eukaryotic Aspartic Proteases / Xin-Li Lin, Ying-Zhang Lin and Jordan Tang
  • 13. Processing of Retroviral Gag Polyproteins: An in Vitro Approach / Carol Carter and Gabriele Zybarth
  • 14. Subsite Preferences of Retroviral Proteinases / Ben M. Dunn, Alla Gustchina, Alexander Wlodawer and John Kay
  • 15. Specificity of Retroviral Proteases: An Analysis of Viral and Nonviral Protein Substrates / Alfredo G. Tomasselli and Robert L. Heinrikson.
  • 16. Rapid Determination of Endoprotease Specificity Using Peptide Mixtures and Edman Degradation Analysis / Frank R. Masiarz and Bruce A. Malcolm
  • 17. Design of Tight-Binding Human Immunodeficiency Virus Type 1 Protease Inhibitors / Joseph P. Vacca
  • 18. Design of Symmetry-Based, Peptidomimetic Inhibitors of Human Immunodeficiency Virus Protease / Dale J. Kempf
  • 19. Advances in Automated Docking Applied to Human Immunodeficiency Virus Type 1 Protease / Michael D. Miller, Robert P. Sheridan, Simon K. Kearsley and Dennis J. Underwood
  • 20. Use of Molecular Dynamics and Free Energy Perturbation Calculations in Anti-Human Immunodeficiency Virus Drug Design / Margaret A. McCarrick and Peter Kollman
  • 21. Qualitative Study of Drug Resistance in Retroviral Protease Using Structural Modeling and Site-Directed Mutagenesis / J. Chris Culberson, Bruce L. Bush and Vinod V. Sardana.

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