|
|
|
|
| LEADER |
00000nam a22000003u 4500 |
| 001 |
b8795663 |
| 003 |
CoU |
| 005 |
20160527234919.3 |
| 006 |
m o d f |
| 007 |
cr ||||||||||| |
| 008 |
160906e20160122||| o| f0|||||eng|d |
| 035 |
|
|
|a (TOE)ost1254484
|
| 035 |
|
|
|a (TOE)1254484
|
| 040 |
|
|
|a TOE
|c TOE
|
| 049 |
|
|
|a GDWR
|
| 072 |
|
7 |
|a 37
|2 edbsc
|
| 086 |
0 |
|
|a E 1.99:1254484
|
| 086 |
0 |
|
|a E 1.99:1254484
|
| 245 |
0 |
0 |
|a Revealing an outward-facing open conformational state in a CLC Cl <sup>–</sup>/H <sup>+</sup> exchange transporter
|h [electronic resource]
|
| 260 |
|
|
|a Washington, D.C. :
|b United States. Department of Energy. Office of Science ;
|a Oak Ridge, Tenn. :
|b distributed by the Office of Scientific and Technical Information, U.S. Department of Energy,
|c 2016.
|
| 300 |
|
|
|a Article No. e11189 :
|b digital, PDF file.
|
| 336 |
|
|
|a text
|b txt
|2 rdacontent.
|
| 337 |
|
|
|a computer
|b c
|2 rdamedia.
|
| 338 |
|
|
|a online resource
|b cr
|2 rdacarrier.
|
| 500 |
|
|
|a Published through SciTech Connect.
|
| 500 |
|
|
|a 01/22/2016.
|
| 500 |
|
|
|a eLife 5 ISSN 2050-084X AM.
|
| 500 |
|
|
|a Han, Wei; Jiang, Tao; Liu, Corey W.; Tajkhorshid, Emad; Mathews, Irimpan I.; Stein, Richard A.; Mchaourab, Hassane S.; Khantwal, Chandra M.; Abraham, Sherwin J.; Chavan, Tanmay S.; Cheng, Ricky C.; Elvington, Shelley M.; Maduke, Merritt.
|
| 500 |
|
|
|a Stanford Univ. School of Medicine, Stanford, CA (United States)
|
| 520 |
3 |
|
|a <p>CLC secondary active transporters exchange Cl<sup>-</sup>for H<sup>+</sup>. Crystal structures have suggested that the conformational change from occluded to outward-facing states is unusually simple, involving only the rotation of a conserved glutamate (Glu<sub>ex</sub>) upon its protonation. Using<sup>19</sup>F NMR, we show that as [H<sup>+</sup>] is increased to protonate Glu<sub>ex</sub>and enrich the outward-facing state, a residue ̃20 Å away from Glu<sub>ex</sub>, near the subunit interface, moves from buried to solvent-exposed. Consistent with functional relevance of this motion, constriction via inter-subunit cross-linking reduces transport. Molecular dynamics simulations indicate that the cross-link dampens extracellular gate-opening motions. In support of this model, mutations that decrease steric contact between Helix N (part of the extracellular gate) and Helix P (at the subunit interface) remove the inhibitory effect of the cross-link. In conclusion, together, these results demonstrate the formation of a previously uncharacterized 'outward-facing open' state, and highlight the relevance of global structural changes in CLC function.</p>
|
| 536 |
|
|
|b AC02-76SF00515.
|
| 536 |
|
|
|b MCA06N060.
|
| 650 |
|
7 |
|a Inorganic, Organic, Physical, And Analytical Chemistry.
|2 edbsc.
|
| 710 |
1 |
|
|a United States.
|b Department of Energy.
|b Office of Science.
|4 spn.
|
| 710 |
1 |
|
|a United States.
|b Department of Energy.
|b Office of Scientific and Technical Information.
|4 dst.
|
| 856 |
4 |
0 |
|u http://www.osti.gov/scitech/biblio/1254484
|z Online Access
|
| 907 |
|
|
|a .b87956639
|b 03-08-23
|c 09-08-16
|
| 998 |
|
|
|a web
|b 09-08-16
|c f
|d m
|e p
|f eng
|g
|h 0
|i 1
|
| 956 |
|
|
|a Information bridge
|
| 999 |
f |
f |
|i 40ac9f63-bb7c-5173-9888-679447a57d16
|s 4afca14c-1af5-5f8a-aabc-50fbd2d42dcc
|
| 952 |
f |
f |
|p Can circulate
|a University of Colorado Boulder
|b Online
|c Online
|d Online
|e E 1.99:1254484
|h Superintendent of Documents classification
|i web
|n 1
|
