Circulating microRNAs : methods and protocols / edited by Nobuyoshi Kosaka.

MicroRNAs (miRNAs) are small non-coding RNAs that regulate various biological phenomena, such as development and homeostasis. The dysregulation of miRNA leads to disease progression, particularly of cancer. In Circulating MicroRNAs: Methods and Protocols, expert researchers in the field detail recen...

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Bibliographic Details
Online Access: Full Text (via Springer)
Other Authors: Kosaka, Nobuyoshi, 1980-
Format: Electronic eBook
Language:English
Published: New York : Humana Press, ©2013.
Series:Methods in molecular biology (Clifton, N.J.) ; v. 1024.
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Summary:MicroRNAs (miRNAs) are small non-coding RNAs that regulate various biological phenomena, such as development and homeostasis. The dysregulation of miRNA leads to disease progression, particularly of cancer. In Circulating MicroRNAs: Methods and Protocols, expert researchers in the field detail recent advances in the isolation, purification and analysis of circulating miRNAs from a variety of sources for research. The book is divided into three main topics. The first section involves the study of secretory miRNAs in cell-cell communication, and the second, the study of circulating miRNAs in body fluids. The last describes the novel techniques used to study circulating miRNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Circulating MicroRNAs: Methods and Protocols seeks to aid scientists in dealing with the recent advances of RNAi technology from the bench to the bedside.
Item Description:Includes index.
Physical Description:1 online resource (xi, 270 pages) : illustrations (some color)
Bibliography:Includes bibliographical references and index.
ISBN:9781627034531
1627034536
1627034528
9781627034524
ISSN:1940-6029 ;
Language:English.