Circulating microRNAs : methods and protocols / edited by Nobuyoshi Kosaka.

MicroRNAs (miRNAs) are small non-coding RNAs that regulate various biological phenomena, such as development and homeostasis. The dysregulation of miRNA leads to disease progression, particularly of cancer. In Circulating MicroRNAs: Methods and Protocols, expert researchers in the field detail recen...

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Bibliographic Details
Online Access: Full Text (via Springer)
Other Authors: Kosaka, Nobuyoshi, 1980-
Format: Electronic eBook
Language:English
Published: New York : Humana Press, ©2013.
Series:Methods in molecular biology (Clifton, N.J.) ; v. 1024.
Subjects:
Table of Contents:
  • Functional analysis of exosomal microRNA in cell-cell communication research / Nobuyoshi Kosaka [and others]
  • Isolation of extracellular nanovesicle microRNA from liver cancer cells in culture / Takayuki Kogure and Tushar Patel
  • Methods of analysis of dendritic cell-derived exosome-shuttle microRNA and its horizontal propagation between dendritic cells / Angela Montecalvo, Adriana T. Larregina, and Adrian E. Morelli
  • Analysis of microRNA and protein transfer by exosomes during an immune synapse / Carolina Villarroya-Beltri
  • Analysis of viral microRNA exchange via exosomes in vitro and in vivo / Frederik J. Verweij [and others]
  • Measurement of precursor miRNA in exosomes from human ESC-cerived mesenchymal stem cells / Tian Sheng Chen and Sai Kiang Lim
  • Analysis of the transfer of circulating microRNA between cells mediated by gap junction / Steven J. Greco and Pranela Rameshwar
  • Isolation of circulating microRNA associated with RNA-binding protein / Andrey Turchinovich, Ludmila Weiz, and Barbara Burwinkel
  • Identification and analysis of circulating exosomal microRNA in human body fluids / Cecilia Lässer
  • Analyzing the circulating microRNAs in exosomes/extracellular vesicles from serum or plasma by qRT-PCR / Leni Moldovan [and others]
  • Direct serum assay for microRNA in cancer patients / Sota Asaga and Dave S.B. Hoon
  • Analysis of microRNA niches : techniques to measure extracellular microRNA and intracellular microRNA in situ / Victoria N. Parikh and Stephen Y. Chan
  • Analysis of circulating microRNA by microarray in liver disease / Yoshiki Murakami and Toshihito Tanahashi
  • Isolation of circulating microRNA in saliva / Alessia Gallo and Ilias Alevizos
  • Purification of RNA from milk whey / Hirohisa Izumi [and others]
  • Circulating microRNAs in the cerebrospinal fluid of patients with brain diseases / Akira Machida, Takuya Ohkubo, and Takanori Yokota
  • Methods of microRNA quantification in urinary sediment / Gang Wang and Cheuk Chun Szeto
  • Circulating microRNA for the identification of forensically relevant body fluids / Erin K. Hanson and Jack Ballantyne
  • Identification of prostate cancer-associated microRNAs in circulation using a mouse model of disease / Luke A. Selth [and others]
  • Combination of extraction reagent and DNA microarray that allows for the detection of global miRNA profiles from serum/plasma / Makiko Ichikawa and Hideo Akiyama
  • Nanopore single-molecule detection of circulating microRNAs / Li-Qun Gu and Yong Wang.