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G protein-coupled receptor signaling in plants : methods and protocols / edited by Mark P. Running.

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Remarkably, while G protein-coupled receptors (GPCRs) are highly prevalent in animals and yeast, very few candidate GPCRs have been identified in plants. In G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols, experts in the field describe techniques used in the study of small GTPa...

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Bibliographic Details
Online Access: Full Text (via Springer)
Main Author: Running, Mark P. (Author, Editor)
Format: eBook
Language:English
Published: New York : Humana Press, 2013.
Series:Methods in molecular biology (Clifton, N.J.) ; v. 1043.
Subjects:
Plant cellular signal transduction > Laboratory manuals.
G proteins > Laboratory manuals.
G proteins.
Plant cellular signal transduction.
Laboratory manuals.
Table of Contents:
  • Topology Assessment, G Protein-Coupled Receptor (GPCR) Prediction, and In Vivo Interaction Assays to Identify Plant Candidate GPCRs
  • Measurement of GTP-Binding and GTPase Activity of Heterotrimeric G Proteins
  • Biochemical Analysis of the Interaction Between Phospholipase D 1 and GTP-Binding Protein -Subunit from Arabidopsis thaliana
  • Analysis of Cell Division and Cell Elongation in the Hypocotyls of Arabidopsis Heterotrimeric G Protein Mutants
  • Aequorin Luminescence-Based Functional Calcium Assay for Heterotrimeric G Proteins in Arabidopsis
  • Methods for Analysis of Disease Resistance and the Defense Response in Arabidopsis
  • Fusarium oxysporum Infection Assays in Arabidopsis
  • Analysis of Unfolded Protein Response in Arabidopsis
  • Functional Analysis of Heterotrimeric G Proteins in Chloroplast Development in Arabidopsis
  • G Protein Signaling in UV Protection: Methods for Understanding the Signals in Young Etiolated Seedlings
  • Functional Analysis of Small Rab GTPases in Cytokinesis in Arabidopsis thaliana
  • In Vivo Localization in Arabidopsis Protoplasts and Root Tissue
  • Analysis of Protein Prenylation and S-Acylation Using Gas Chromatography Coupled Mass Spectrometry
  • In Vitro Myristoylation Assay of Arabidopsis Proteins
  • Assaying Protein S-Acylation in PlantIn Vitro Prenylation Assay of Arabidopsis Proteins.
  • Topology Assessment, G Protein-Coupled Receptor (GPCR) Prediction, and In Vivo Interaction Assays to Identify Plant Candidate GPCRs
  • Measurement of GTP-Binding and GTPase Activity of Heterotrimeric Gα Proteins
  • Biochemical Analysis of the Interaction Between Phospholipase Dα1 and GTP-Binding Protein α-Subunit from Arabidopsis thaliana
  • Analysis of Cell Division and Cell Elongation in the Hypocotyls of Arabidopsis Heterotrimeric G Protein Mutants
  • Aequorin Luminescence-Based Functional Calcium Assay for Heterotrimeric G Proteins in Arabidopsis
  • Methods for Analysis of Disease Resistance and the Defense Response in Arabidopsis
  • Fusarium oxysporum Infection Assays in Arabidopsis
  • Analysis of Unfolded Protein Response in Arabidopsis
  • Functional Analysis of Heterotrimeric G Proteins in Chloroplast Development in Arabidopsis
  • G Protein Signaling in UV Protection: Methods for Understanding the Signals in Young Etiolated Seedlings
  • Functional Analysis of Small Rab GTPases in Cytokinesis in Arabidopsis thaliana
  • In Vivo Localization in Arabidopsis Protoplasts and Root Tissue
  • Analysis of Protein Prenylation and S-Acylation Using Gas Chromatography-Coupled Mass Spectrometry
  • In Vitro Myristoylation Assay of Arabidopsis Proteins
  • Assaying Protein S-Acylation in PlantIn Vitro Prenylation Assay of Arabidopsis Proteins.

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