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DNA microarrays [electronic resource] / edited by Alan Kimmel & Brian Oliver.

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Modern DNA microarray technologies have evolved over the past 25 years to the point where it is now possible to take many million measurements from a single experiment. These two volumes, Parts A & B in the Methods in Enzymology series provide methods that will shepard any molecular biologist th...

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Bibliographic Details
Online Access: Full Text (via ScienceDirect)
Other Authors: Kimmel, Alan R., Oliver, Brian, 1959-
Format: Electronic eBook
Language:English
Published: Amsterdam ; San Diego : Elsevier/Academic Press, ©2006.
Series:Methods in enzymology ; v. 410-411.
Subjects:
DNA microarrays.
DNA.
Table of Contents:
  • pt. A. Array platforms and wet-bench protocols
  • pt. B. Databases and statistics.
  • Section I Array Platforms
  • Chapter 1: The Affymetrix GeneChipreg Platform: An Overview
  • Introduction
  • GeneChip Microarrays, a Flexible Platform
  • Array Manufacturing
  • Array Design
  • Target Preparation
  • GeneChip Instrument Components and Associated Assay Steps
  • Image and Data Analysis
  • Current Applications
  • Advancing the Future of Genomics
  • Acknowledgments
  • References
  • Chapter 2: The Agilent In Situ-Synthesized Microarray Platform
  • Introduction
  • Technology
  • Applications
  • Methods Descriptions
  • Array Design
  • Sample Isolation, Labeling, and Quality Control
  • Array Hybridization and Scanning
  • Data Extraction
  • Array Design
  • Process Description
  • Sample Isolation, Labeling, and Quality Control
  • Array Hybridization and Scanning
  • Data Extraction
  • References
  • Chapter 3: Illumina Universal Bead Arrays
  • Introduction
  • Material and Methods
  • Results and Discussion
  • Conclusion
  • Acknowledgments
  • References
  • Chapter 4: Microarray Oligonucleotide Probes
  • The Case for Oligonucleotide Probes
  • Considerations for Oligonucleotide Probe Design
  • Microarray Production and Hybridization Protocols
  • Practical Considerations in Probe Sequence Design, a Case Study
  • Employment and Postprocessing
  • Conclusion
  • Locations of Probe Sequence Target Regions: Discrimination of Highly Similar Targets
  • In Situ Synthesis vs Deposition of Presynthesized Oligonucleotides
  • Thermodynamic Modeling of Microarray Probe Hybridization
  • Outlook
  • Supplement
  • Acknowledgments
  • References
  • Chapter 5: Automated Liquid Handling and High-Throughput Preparation of Polymerase Chain Reaction-Amplified DNA for Microarray Fabrication
  • Introduction
  • Overview
  • Methods
  • Summary
  • Acknowledgments
  • References
  • Chapter 6: The.

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