Simultaneous cryo X-ray ptychographic and fluorescence microscopy of green algae [electronic resource]
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| Online Access: |
Online Access (via OSTI) |
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| Format: | Government Document Electronic eBook |
| Language: | English |
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Bethesda, Md. : Oak Ridge, Tenn. :
National Institutes of Health (U.S.) ; distributed by the Office of Scientific and Technical Information, U.S. Department of Energy,
2015.
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| Abstract: | Trace metals play important roles in normal and in disease-causing biological functions. X-ray fluorescence microscopy reveals trace elements with no dependence on binding affinities (unlike with visible light fluorophores) and with improved sensitivity relative to electron probes. However, X-ray fluorescence is not very sensitive for showing the light elements that comprise the majority of cellular material. Here we show that X-ray ptychography can be combined with fluorescence to image both cellular structure and trace element distribution in frozen-hydrated cells at cryogenic temperatures, with high structural and chemical fidelity. Ptychographic reconstruction algorithms deliver phase and absorption contrast images at a resolution beyond that of the illuminating lens or beam size. Using 5.2-keV X-rays, we have obtained sub-30-nm resolution structural images and ̃90-nm-resolution fluorescence images of several elements in frozen-hydrated green algae. Finally, this combined approach offers a way to study the role of trace elements in their structural context. |
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| Item Description: | Published through SciTech Connect. 02/09/2015. "124620" Proceedings of the National Academy of Sciences of the United States of America 112 8 ISSN 0027-8424 AM. Junjing Deng; David J. Vine; Si Chen; Youssef S. G. Nashed; Qiaoling Jin; Nicholas W. Phillips; Tom Peterka; Rob Ross; Stefan Vogt; Chris J. Jacobsen. |