Aqueous two-phase systems for bioprocess development for the recovery of biological products / Marco Rito-Palomares, Jorge Benavides, editors.
This comprehensive and unique text presents a full overview of downstream processing useful for those new to the concept as well as professionals with experience in the area. The history and theoretical principles of Aqueous Two-Phase Systems (ATPS) are covered in depth. Information on ATPS characte...
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Full Text (via Springer) |
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| Other Authors: | , |
| Format: | eBook |
| Language: | English |
| Published: |
Cham :
Springer,
2017.
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| Series: | Food engineering series ;
v. 1. |
| Subjects: |
Table of Contents:
- Preface; Contents; Contributors; 1: General Concepts and Definitions of Aqueous Two-Phase Systems; 1.1 Introduction; 1.2 Binodal Curve, Tie-Line Length, and Volume Ratio; 1.3 General Classification of ATPS; 1.3.1 Polymer-Polymer Systems; 1.3.2 Polymer-Salt Systems; 1.3.3 Alcohol-Salt Systems; 1.3.4 Micellar Systems; 1.3.5 Ionic Liquid-Based Systems; 1.4 Definitions of Parameters of ATPS: Partition Coefficient, Selectivity, Recovery Yield, and Purity; 1.4.1 Partition Coefficient; 1.4.2 Selectivity; 1.4.3 Recovery Yield; 1.4.4 Purity.
- 1.5 Factors that Affect Partitioning in ATPS: Molecular Weight, Electrochemical Interactions and pH, Hydrophobicity, and Affinity1.5.1 Molecular Weight or Size; 1.5.2 Electrochemical Interactions and pH; 1.5.3 Hydrophobicity; 1.5.4 Affinity; 1.6 Concluding Remarks; References; 2: Characterization of Aqueous Two-Phase Systems and Their Potential New Applications; 2.1 Introduction; 2.2 Aqueous Two-Phase System Characterization; 2.3 Solute Partition Characterization; 2.4 Novel Operational Strategies and Potential New Applications for Aqueous Two-Phase Systems; 2.5 Concluding Remarks.
- 3.3.3 Recovery of Invertase from Wasted Brewer's Yeast3.4 ATPS Strategies for the Recovery of PEGylated Proteins; 3.5 Protein Refolding Explorations in ATPS; 3.6 Concluding Remarks; References; 4: Aqueous Two-Phase Systems for the Recovery of Bioparticles; 4.1 Introduction; 4.2 Partitioning of Whole Cells, Stem Cells, and Cellular Components in ATPS; 4.2.1 Blood Components; 4.2.2 Stem Cells; 4.2.3 Other Cell Types; 4.3 Cell Components; 4.4 Partition of Viral Particles: Toward New Therapeutic Strategies; 4.5 Nucleic Acids Recovery in ATPS: Current Innovations.
- 4.6 Macroparticle Recovery with ATPS: Challenges and Future Trends4.7 Concluding Remarks; References; 5: Aqueous Two-Phase System Strategies for the Recovery and Partial Purification of Bioactive Low Molecular Weight Compounds; 5.1 Introduction; 5.2 Evolution of the Use of Aqueous Two-Phase Systems for the Recovery and Partial Purification of Low Molecular Weight Compounds; 5.3 Selected Studies on the Extraction, Recovery, and Partial Purification of Low Molecular Weight Compounds Using Aqueous Two-Phase Systems; 5.3.1 Polymer-Polymer and Polymer-Salt ATPS; 5.3.2 Alcohol-Salt ATPS.