Gene targeting protocols / edited by Eric B. Kmiec.
In Gene Targeting Protocols, a panel of innovative investigators present in readily reproducible detail the latest techniques for gene replacement, gene knockout, and gene repair in both animals and plants. These expert researchers review the best gene transfer vehicles (liposomes, PEI, and EPD), di...
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| Online Access: |
Full Text (via EBSCO) |
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| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
©2000.
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| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 133. |
| Subjects: |
Table of Contents:
- Nucleic acid transfer using cationic lipids / Natasha J. Caplen
- Optimizing polyethylenimine-based gene transfer into mammalian brain for analysis of promoter regulation and protein function / Barbara A. Demeneix, Mohamed Ghorbel, Daniel Goula
- Gene transfer and drug delivery by electronic pulse delivery : a nonviral delivery approach / Xi Zhao
- Strategies for improving the frequency and assessment of homologous recombination / Nancy Smyth Templeton
- Effective gene transfer using viral vectors based on SV40 / David S. Strayer
- Rapid generation of isogenic mammalian cell lines expressing recombinant transgenes by use of cre recombinase / Brude D. Bethke, Brian Sauer
- Site-directed alteration of genomic DNA by small-fragment homologous replacement / Kaarin K. Goncz, Dieter C. Gruenert
- Mutation correction by homologous recombination with an adenovirus vector / Ayumi Fujita-Kusano [and others]
- Site-specific targeting of DNA plasmids to chromosome 19 using AAV cis and trans sequences / Samuel M. Young, Jr., Weidong Xiao, Richard Jude Samulski
- Adeno-associated virus based gene therapy in skeletal muscle / Richard J. Bartlett, Jesica M. McCue
- Rapid establishment of myeloma cell lines expressing fab(tac)-protamine, a targetable protein vector, directed against high-affinity [alpha]-chain of human interleukin-2 receptor / Sun U. Song, Wayne A. Marasco
- EBV-derived episomes to probe chromatin structure and gene expression in human cells / Juliette Fivaz [and others]
- Triplex-directed site-specific genome modification / Karen M. Vasquez, John H. Wilson
- Use of quantitative ligation-mediated polymerase chain reaction to detect gene targeting by alkylating oligodeoxynucleotides / Howard B. Gamper [and others]
- Gene targeting in plants via site-directed mutagenesis / Peter B. Kipp [and others]
- Antisense oligonucleotides as modulators of pre-mRNA splicing / Halina Sierakowska, Sudhir Agrawal, Ryszard Kole.
- 9. Site-specific targeting of DNA plasmids to chromosome 19 using AAV cis and trans sequences / Samuel M. Young Jr., Weidong Xiao & Richard Jude Samulski
- 10. Adeno-associated virus based gene therapy in skeletal muscle / Richard J. Bartlett & Jesica M. McCue
- 11. Rapid establishment of myeloma cell lines expressing fab(tac)-protamine, a targetable protein vector, directed against high-affinity α-chain of human interleukin-2 receptor / Sun U. Song & Wayne A. Marasco
- 12. EBV-derived episomes to probe chromatin structure and gene expression in human cells / Juliette Fivaz, M. Chiara Bassi, Stéphane Pinaud, Larry Richman, Melanie Price & Jovan Mirkovitch
- 13. Triplex-directed site-specific genome modification / Karen M. Vasquez & John H. Wilson
- 14. Use of quantitative ligation-mediated polymerase chain reaction to detect gene targeting by alkylating oligodeoxynucleotides / Howard B. Gamper, Irina Afonina, Evgeniy Belousov, Michael W. Reed & Mikhail A. Pdyminogin
- 15. Gene targeting in plants via site-directed mutagenesis / Peter B. Kipp, Joyce M. Van Eck, Peter R. Beetham & Gregory D. May
- 16. Antisense oligonucleotides as modulators of pre-mRNA splicing / Halina Sierakowska, Sudhir Agrawal & Ryszard Kole.