DNA replication : methods and protocols / edited by Sonya Vengrova, Jacob Dalgaard.
Updated and revised, this thorough volume covers a range of methods focusing on systems, including mammalian, yeast, bacterial and archaeal. This second edition of DNA Replication: Methods and Protocols describes approaches to analyze whole genomes to single molecules, as well as both in vivo and in...
Saved in:
| Online Access: |
Full Text (via Springer) |
|---|---|
| Other Authors: | , |
| Format: | Electronic eBook |
| Language: | English |
| Published: |
New York, NY :
Humana Press,
2015.
|
| Edition: | Second edition. |
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 1300. |
| Subjects: |
MARC
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| 245 | 0 | 0 | |a DNA replication : |b methods and protocols / |c edited by Sonya Vengrova, Jacob Dalgaard. |
| 250 | |a Second edition. | ||
| 264 | 1 | |a New York, NY : |b Humana Press, |c 2015. | |
| 300 | |a 1 online resource (x, 295 pages) : |b illustrations (some color) | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 490 | 1 | |a Methods in Molecular Biology, |x 1064-3745 ; |v 1300 | |
| 500 | |a Includes index. | ||
| 588 | 0 | |a Online resource; title from PDF title page (SpringerLink, viewed May 8, 2015). | |
| 505 | 0 | |a A High-Throughput Confocal Fluorescence Microscopy Platform to Study DNA Replication Stress in Yeast Cells -- Microscopy Techniques to Examine DNA Replication in Fission Yeast -- High-Resolution Analysis of Mammalian DNA Replication Units -- Analysing the Dynamics of DNA Replication In Mammalian Cells Using DNA Combing -- Measuring DNA Content by Flow Cytometry in Fission Yeast -- Incorporation of Thymidine Analogs for Studying Replication Kinetics In Fission Yeast -- Edu Incorporation for FACS and Microscopy Analysis of DNA Replication in Budding Yeast -- Determination Of Deoxyribonucleoside Triphosphate Concentrations in Yeast Cells by Strong Anion-Exchange High-Performance Liquid Chromatography Coupled with Ultraviolet Detection -- Measuring Ribonucleotide Incorporation Into DNA In Vitro and In Vivo -- Detection And Sequencing Of Okazaki Fragments In S. Cerevisiae -- Chip-Seq To Analyze The Binding Of Replication Proteins To Chromatin -- Chromatin Immunoprecipitation To Detect DNA Replication And Repair Factors -- Molecular Genetic Methods To Study DNA Replication Protein Function In Haloferax Volcanii, A Model Archaeal Organism -- Single-Molecule Observation Of Prokaryotic DNA Replication -- Analysing the Response to Dysfunction Replication Forks Using the RTS1 Barrier System in Fission Yeast -- Purification of Restriction Fragments Containing Replication Intermediates from Complex Genomes for 2-D Gel Analysis -- Isolation of Restriction Fragments Containing Origins of Replication from Complex Genomes. | |
| 520 | |a Updated and revised, this thorough volume covers a range of methods focusing on systems, including mammalian, yeast, bacterial and archaeal. This second edition of DNA Replication: Methods and Protocols describes approaches to analyze whole genomes to single molecules, as well as both in vivo and in vitro experiments. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Replication: Methods and Protocols, Second Edition provides a collections of methods intended for newcomers to this research field and for established laboratories. | ||
| 504 | |a Includes bibliographical references and index. | ||
| 650 | 0 | |a DNA replication |v Laboratory manuals. | |
| 650 | 0 | |a DNA replication. | |
| 650 | 7 | |a DNA replication |2 fast | |
| 655 | 7 | |a Laboratory manuals |2 fast | |
| 700 | 1 | |a Vengrova, Sonya, |e editor. | |
| 700 | 1 | |a Dalgaard, Jacob Z., |e editor. | |
| 776 | 0 | 8 | |i Print version: |t DNA replication. |b Second edition. |d New York, NY : Humana Press, 2015 |w (DLC) 2015935079 |
| 830 | 0 | |a Methods in molecular biology (Clifton, N.J.) ; |v v. 1300. |x 1064-3745 | |
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