Cell-penetrating peptides : methods and protocols / edited by Ülo Langel.

Divided into three parts this volume summarizes the most important areas of Cell-Penetrating Peptides (CPP) research . Partone briefly presents the historical background of CPP studies and the classifications of the available CPPs, and then summarizes the approaches for prediction of novel CPPs. Par...

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Bibliographic Details
Online Access: Full Text (via Springer)
Other Authors: Langel, Ülo (Editor)
Other title:Cell-penetrating peptides (Humana Press)
Format: Electronic eBook
Language:English
Published: New York : Humana Press : Springer, [2015]
Edition:Second edition.
Series:Methods in molecular biology (Clifton, N.J.) ; v. 1324.
Springer protocols (Series)
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MARC

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504 |a Includes bibliographical references and index. 
505 0 0 |t Classes of cell-penetrating peptides /  |r Margus Pooga and Ülo Langel --  |t Penetratin story : an overview /  |r Edmond Dupont, Alain Prochiantz, and Alain Joliot --  |t Prediction of cell-penetrating peptides /  |r Mattias Hällbrink and Mati Karelson --  |t Computer-aided virtual screening and designing of cell- penetrating peptides /  |r Ankur Gautam, Kumardeep Chaudhary, Rahul Kumar, and Gajendra Pal Singh Raghava --  |t Investigating membrane interactions and structures of CPPs /  |r Fatemeh Madani and Astrid Gräslund --  |t Determining the effects of membrane-interacting peptides on membrane integrity /  |r William C. Wimley --  |t Study of CPP mechanisms by mass spectrometry /  |r Sandrine Sagan, Chérine Bechara, and Fabienne Burlina --  |t Methods to study the role of the glycocalyx in the uptake of cell-penetrating peptides /  |r Samuel Schmidt, Rike Wallbrecher, Toin H. van Kuppevelt, and Roland Brock --  |t Toxicity, immunogenicity, uptake, and kinetics methods for CPPs /  |r Julia Uusna, Kent Langel, and Ülo Langel --  |t Unraveling the mechanisms of peptide-mediated delivery of nucleic acids using electron microscopy /  |r Helerin Margus, Carmen Juks, and Margus Pooga --  |t SCARA involvement in the uptake of nanoparticles formed by cell-penetrating peptides /  |r Henrik Helmfors, Staffan Lindberg, and Ülo Langel --  |t Protein mimicry and the design of bioactive Cell- penetrating peptides /  |r John Howl and Sarah Jones --  |t Pepducins and other lipidated peptides as mechanistic probes and therapeutics /  |r Ping Zhang, Lidija Covic, and Athan Kuliopulos --  |t Identification and characterization of homing peptides using in vivo peptide phage display /  |r Maija Hyvönen and Pirjo Laakkonen --  |t Antimicrobial and antiviral applications of cell- penetrating peptides /  |r Kalle Pärn, Elo Eriste, and Ülo Langel --  |t Visualizing actin architectures in cells incubated with cell-penetrating peptides /  |r Lin He, Peter D. Watson, and Arwyn T. Jones --  |t Cell-penetrating peptides as carriers for transepithelial drug delivery in vitro /  |r Stine Rønholt, Mie Kristensen, and Hanne Mørck Nielsen --  |t Pathway toward tumor cell-selective CPPs? /  |r Isabel D. Alves, Manon Carré, and Solange Lavielle --  |t PepFects and nickFects for the intracellular delivery of nucleic acids /  |r Piret Arukuusk, Ly Pärnaste, Mattias Hällbrink, and Ülo Langel --  |t In vitro assays to assess exon skipping in duchenne muscular dystrophy /  |r Prisca Boisguerin, Liz O'Donovan, Michael J. Gait, and Bernard Lebleu --  |t Applications of ApoB LDLR-binding domain approach for the development of CNS-penetrating peptides for alzheimer's disease /  |r Eliezer Masliah and Brian Spencer --  |t CPP-based delivery system for in vivo gene delivery /  |r Kaido Kurrikoff, Kadi-Liis Veiman, and Ülo Langel --  |t Application of CPPs for brain delivery /  |r Artita Srimanee, Jakob Regberg, and Ülo Langel --  |t Intracellular delivery of nanoparticles with cell penetrating peptides /  |r Giuseppina Salzano and Vladimir P. Torchilin --  |t Multifunctional oligoaminoamides for the receptor-specific delivery of therapeutic RNA /  |r Judith Weber, Ulrich Lächelt, and Ernst Wagner --  |t Cell penetrating peptides for chemical biological studies /  |r Ikuhiko Nakase, Toshihide Takeuchi, and Shiroh Futaki --  |t Experiences with CPP-based self assembling peptide systems for topical delivery of botulinum toxin /  |r Jane Lee, Phil Kennedy, and Jacob M. Waugh --  |t Applications of CPPs in genome modulation of plants /  |r Alicja Ziemienowicz, Jordan Pepper, and François Eudes --  |t DNA transfer into animal cells using stearylated CPP based Transfection Reagent /  |r Kristiina Karro, Tiiu Männik, Andres Männik, and Mart Ustav --  |t Live cell genomics : cell-specific transcriptome capture in live tissues and cells /  |r Thomas J. Bell and James Eberwine --  |t Live cell genomics : RNA exon-specific RNA-binding protein isolation /  |r Thomas J. Bell and James Eberwine. 
520 |a Divided into three parts this volume summarizes the most important areas of Cell-Penetrating Peptides (CPP) research . Partone briefly presents the historical background of CPP studies and the classifications of the available CPPs, and then summarizes the approaches for prediction of novel CPPs. Parttwo mainly describes the methods for studies of naked CPPs, that is, CPPs without conjugated cargos. Last but not least part threepresents a representative and brief summary of functionality issues of CPPs, both in vitro and in vivo. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use, Cell-Penetrating Peptides: Methods and Protocols, Second Edition hopes to raise relevant questions for further development. 
650 0 |a Peptides  |x Physiological transport. 
650 0 |a Drug carriers (Pharmacy) 
650 0 |a Cells  |x Permeability. 
650 7 |a Cells  |x Permeability  |2 fast 
650 7 |a Drug carriers (Pharmacy)  |2 fast 
650 7 |a Peptides  |x Physiological transport  |2 fast 
700 1 |a Langel, Ülo,  |e editor. 
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830 0 |a Springer protocols (Series) 
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