Synthetic mRNA : production, introduction into cells and physiological consequences / edited by Robert E. Rhoads.
This volume presents detailed laboratory protocols for in vitro synthesis of mRNA with favorable properties, its introduction into cells by a variety of techniques, and the measurement of physiological and clinical consequences such as protein replacement and cancer immunotherapy. Synthetic techniqu...
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Full Text (via Springer) |
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| Format: | Electronic eBook |
| Language: | English |
| Published: |
New York :
Humana Press : Springer,
©2016.
|
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 1428. |
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Table of Contents:
- Synthetic mRNA: Production, Introduction into Cells, and Physiological Consequences
- Synthetic Capped mRNAs for Cap-Specific Photo-Crosslinking Experiments
- Enzymatic Modification of 5'-Capped RNA and Subsequent Labeling by Click Chemistry
- Preparation of Functional, Fluorescently Labeled mRNA Capped with Anthraniloyl-m7GpppG
- Intronless b-Globin Reporter: A Tool for Studying Nuclear RNA Stability Elements
- Synthetic mRNA with Superior Properties that Mimics the Intracellular Fates of Natural Histone mRNA
- Engineering WT1-Encoding mRNA to Increase Translational Efficiency in Dendritic Cells
- Electroporation of Alphavirus RNA Translational Reporters into Fibroblastic and Myeloid Cells as a Tool to Study the Innate Immune System
- GMP-Grade mRNA Electroporation of Dendritic Cells for Clinical Use
- Large Scale mRNA Transfection of Dendritic Cells by Electroporation in Continuous Flow Systems
- FLT3 Ligand as a Molecular Adjuvant for Naked RNA Vaccines
- Transfecting Human Monocytes with RNA
- In Vitro Synthesis, Delivery and Bioavailability of Exogenous mRNA in Gene Transfer Mediated byPiggyBac Transposition
- Transfection of Human Keratinocytes with Nucleoside-Modified mRNA Encoding CPD-Photolyase to Repair DNA Damage
- Delivery of Synthetic mRNA Encoding FOXP3 Antigen into Dendritic Cells for Inflammatory Breast Cancer Immunotherapy
- Immune Monitoring using mRNA-Transfected Dendritic Cells
- Transfection of Tumor-Infiltrating T Cells with mRNA Encoding CXCR2
- mRNA Electroporation of Dendritic Cells with WT1, Survivin and TriMix (A Mixture of caTLR4, CD40L, and CD70)
- Redirecting T Cell Specificity Using T Cell Receptor Messenger RNA Electroporation
- Measuring Hematocrit in Mice Injected with In Vitro-Transcribed Erythropoietin mRNA
- Genetic Modification of Human Pancreatic Progenitor Cells through Modified mRNA.
- Synthetic mRNA : production, introduction into cells, and physiological consequences / Robert E. Rhoads
- Synthetic capped mRNAs for cap-specific photo-cross-linking experiments / Joanna Kowalska, Franck Martin, and Jacek Jemielity
- Enzymatic modification of 50-capped RNA and subsequent labeling by click chemistry / Josephin M. Holstein, Daniela Stummer, and Andrea Rentmeister
- Preparation of functional, fluorescently labeled mRNA capped with anthraniloyl-m7GpppG / Artem V. Domashevski [and others]
- Intronless β-Globin reporter : a tool for studying nuclear RNA stability elements / Jessica A. Brown and Joan A. Steitz
- Synthetic mRNA with superior properties that mimics the intracellular fates of natural histone mRNA / Wei Su [and others]
- Engineering WT1-encoding mRNA to increase translational efficiency in dendritic cells / Daphne Benteyn [and others]
- Electroporation of alphavirus RNA translational reporters into fibroblastic and myeloid cells as a tool to study the innate immune system / Christina L. Gardner [and others]
- GMP-grade mRNA electroporation of dendritic cells for clinical use / Judith Derdelinckx, Zwi N. Berneman, and Nathalie Cools
- Large-scale mRNA transfection of dendritic cells by electroporation in continuous flow systems / David Selmeczi
- FLT3 ligand as a molecular adjuvant for naked RNA vaccines / Sebastian Kreiter [and others]
- Transfecting human monocytes with RNA / Jens Dannull and Smita K. Nair
- In vitro synthesis, delivery, and bioavailability of exogenous mRNA in gene transfer mediated by PiggyBac transposition / Solenne Bire, Nicole Ishac, and Florence Rouleux-Bonnin
- Transfection of human keratinocytes with nucleoside-modified mRNA encoding CPD-photolyase to repair DNA damage / Gabor Boros [and others]
- Delivery of synthetic mRNA encoding FOXP3 antigen into dendritic cells for inflammatory breast cancer immunotherapy / Gayathri R. Devi and Sritama Nath
- Immune monitoring using mRNA-transfected dendritic cells / Troels Holz Borch, Inge Marie Svane, and Ozcan Met
- Transfection of tumor-infiltrating T cells with mRNA Encoding CXCR2 / Manja Idorn [and others]
- mRNA electroporation of dendritic cells with WT1, survivin, and TriMix (a mixture of caTLR4, CD40L, and CD70) / An Coosemans [and others]
- Redirecting T cell specificity using T cell receptor messenger RNA electroporation / Sarene Koh, Noriko Shimasaki, and Antonio Bertoletti
- Measuring hematocrit in mice injected with in vitro-transcribed erythropoietin mRNA / Azita Josefine Mahiny and Katalin Kariko
- Genetic modification of human pancreatic progenitor cells through modified mRNA / Song Lu [and others].