Metagenomics : methods and protocols / edited by Wolfgang R. Streit, Rolf Daniel.
This second edition explores up-to-date tools in various function-based technologies currently used in metagenomics. The chapters in this book discuss all of the working steps involved in these technologies, such as: DNA isolation from soils and marine samples followed by the construction and screen...
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| Online Access: |
Full Text (via Springer) |
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| Other Authors: | , |
| Format: | Electronic eBook |
| Language: | English |
| Published: |
New York :
Humana Press : Springer,
[2017]
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| Edition: | Second edition. |
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 1539. Springer protocols (Series) |
| Subjects: |
Table of Contents:
- Construction of small-insert and large-insert metagenomic libraries / Carola Simon and Rolf Daniel
- Extraction of total DNA and RNA from marine filter samples and generation of a cDNA as universal template for marker gene studies / Dominik Schneider, Franziska Wemheuer, Birgit Pfeiffer, and Bernd Wemheuer
- Construction and screening of marine metagenomic large insert libraries / Nancy Weiland-Bräuer, Daniela Langfeldt, and Ruth A. Schmitz
- Constructing and screening a metagenomic library of a cold and alkaline extreme environment / Mikkel A. Glaring, Jan K. Vester, and Peter Stougaard
- DNA-, RNA-, and protein-based stable-isotope probing for high-throughput biomarker analysis of active microorganisms / Eleanor Jameson, Martin Taubert, Sara Coyotzi, Yin Chen, Özge Eyice, Hendrik Schäfer, J. Colin Murrell, Josh D. Neufeld, and Marc G. Dumont
- Assessing bacterial and fungal diversity in the plant endosphere / Bernd Wemheuer and Franziska Wemheuer
- Shotgun metagenomic sequencing analysis of soft-rot Enterobacteriaceae in polymicrobial communities / James Doonan, Sandra Denman, James E. McDonald, and Peter N. Golyshin
- Cloning and expression of metagenomic DNA in Streptomyces lividans and subsequent fermentation for optimized production / Yuriy Rebets, Jan Kormanec, Andriy Lutzhetskyy, Kristel Bernaerts, and Jozef Anné
- Degradation network reconstruction guided by metagenomic data / Rafael Bargiela and Manuel Ferrer
- Novel tools for the functional expression of metagenomic DNA / Nadine Katzke, Andreas Knapp, Anita Loeschcke, Thomas Drepper, and Karl-Erich Jaeger
- Microtiter plate-based assay to screen for active and stereoselective hydrolytic enzymes in enzyme libraries / Dominique Böttcher, Patrick Zägel, Marlen Schmidt, and Uwe T. Bornscheuer
- Screening for cellulase encoding clones in metagenomic libraries / Nele Ilmberger and Wolfgang R. Streit
- Liquid phase multiplex high-throughput screening of metagenomic libraries using p-nitrophenyl-linked substrates for accessory lignocellulosic enzymes / Mariette Smart, Robert J. Huddy, Don A. Cowan, and Marla Trindade
- Screening glycosyltransferases for polyphenol modifications / Nele Ilmberger and Ulrich Rabausch
- Methods for the isolation of genes encoding novel PHA metabolism enzymes from complex microbial communities / Jiujun Cheng, Ricardo Nordeste, Maria A. Trainer, and Trevor C. Charles
- Function-based metagenomic library screening and heterologous expression strategy for genes encoding phosphatase activity / Genis A. Castillo Villamizar, Heiko Nacke, and Rolf Daniel
- Activity-based screening of metagenomic libraries for hydrogenase enzymes / Nicole Adam and Mirjam Perner
- Screening for N-AHSL-based-signaling interfering enzymes / Stéphane Uroz and Phil M. Oger
- Mining microbial signals for enhanced biodiscovery of secondary metabolites / F. Jerry Reen, Jose A. Gutiérrez-Barranquero, and Fergal O'Gara.