Enzyme stabilization and immobilization : methods and protocols / edited by Shelley D. Minteer.
This volume introduces the reader to the field of enzyme stabilization and the different theories of enzyme stabilization, including the use of immobilization as a stabilization technique. The first part of the book focuses on protocols for enzyme stabilization in solutions including liposome format...
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| Online Access: |
Full Text (via Springer) |
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| Other Authors: | |
| Format: | Electronic eBook |
| Language: | English |
| Published: |
New York, N.Y. :
Humana Press : Springer,
©2017.
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| Edition: | 2nd ed. |
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 1504. Springer protocols (Series) |
| Subjects: |
| Summary: | This volume introduces the reader to the field of enzyme stabilization and the different theories of enzyme stabilization, including the use of immobilization as a stabilization technique. The first part of the book focuses on protocols for enzyme stabilization in solutions including liposome formation, micelle introduction, crosslinking, and additives. The second part of the book discusses protocols for enzyme stabilization during enzyme immobilization, including common techniques like sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Protocols for a variety of enzymes are shown, but the enzymes are chosen as examples to show that these protocols can be used for both enzymes of biological importance, as well as enzymes of industrial importance. The final part details spectroscopic protocols, methods, and assays for studying the effectiveness of the enzyme stabilization and immobilization strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Enzyme Stabilization and Immobilization: Methods and Protocols, Second Edition provides molecular biologists, biochemists, and biomedical and biochemical engineers with the state-of-the-art technical information required to effectively stabilize their enzyme of interest in a variety of environments (i.e., harsh temperature, pH, or solvent conditions). |
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| Item Description: | Title from PDF title page (SpringerProtocol, viewed October 24, 2016). |
| Physical Description: | 1 online resource (x, 234 pages) : illustrations (some color) |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: | 9781493964994 1493964992 1493964976 9781493964970 |
| ISSN: | 1064-3745 ; |