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Fast detection of DNA damage : methods and protocols / edited by Vladimir V. Didenko.

This volume presents a comprehensive collection of quick assays for the detection of nuclear and mitochondrial DNA damage and its effects in live and fixed cells and tissues, and in bacterial genomes. Although, such rapid techniques are in demand in the "research trenches" they are not cov...

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Bibliographic Details
Online Access: Full Text (via Springer)
Other Authors: Didenko, Vladimir V. (Editor)
Format: Electronic eBook
Language:English
Published: New York, NY : Humana Press : Springer, [2017]
Series:Methods in molecular biology (Clifton, N.J.) ; v. 1644.
Subjects:
DNA damage.
DNA damage
Laboratory manuals
Table of Contents:
  • Express FRET Labeling and Analysis of Phagocytic Clearance
  • Rapid Assessment of Genotoxicity by Flow Cytometric Detection of Cell Cycle Alterations
  • Ultrasound Imaging of DNA-Damage Effects in Live Cultured Cells and in Brain Tissue
  • Ultrasound Imaging of Apoptosis: Spectroscopic Detection of DNA-Damage Effects In Vivo
  • Fluorochrome-Labeled Inhibitors of Caspases: Expedient In Vitro and In Vivo Markers of Apoptotic Cells for Rapid Cytometric Analysis
  • The Fast-halo Assay for the Detection of DNA Aamage
  • Rapid Detection of Bacterial Susceptibility or Resistance to Quinolones
  • Rapid Detection of Apoptosis in Cultured Mammalian Cells
  • Quick Detection of DNase II-type Breaks in Formalin-Fixed Tissue Sections
  • Express?-H2AX Immunocytochemical Detection of DNA damage
  • Rapid detection of?-H2AX by Flow Cytometry in Cultured Mammalian Cells Firsanov
  • Rapid Detection of DNA Strand Breaks in Apoptotic Cells by Flow- and Image- Cytometry
  • Fast Micromethod: Determination of DNA Integrity in Cell Suspensions and in Solid Tissues
  • Quantification of DNA Damage and Repair in Mitochondrial, Nuclear, and Bacterial Genomes by Real-Time PCR
  • Zebra Tail Amplification: Accelerated Detection of Apoptotic Blunt Ended DNA Breaks by In Situ Ligation
  • Twelve Gel Comet Assay Format for Quick Examination of DNA Damage and Repair
  • Immunofluorescence Analysis of?-H2AX Foci in Mammalian Fibroblasts at Different Phases of the Cell Cycle
  • RAPD-PCR as a Rapid Approach for the Evaluation of Genotoxin-Induced Damage to Bacterial DNA
  • Rapid Detection of?-H2Av Foci in ex vivo MMS-treated Drosophila Imaginal Discs.
  • Express FRET labeling and analysis of phagocytic clearance / Vladimir V. Didenko
  • Rapid assessment of genotoxicity by flow cytometric detection of cell sycle alterations / Nevenka Bihari
  • Ultrasound imaging of DNA-damage effects in live cultured cells and in brain tissue / Hadi Tadayyon, Mehrdad J. Gangeh, Roxana Vlad, Michael C. Kolios, and Gregory J. Czarnota
  • Ultrasound imaging of apoptosis : spectroscopic detection of DNA-damage effects in vivo / Hadi Tadayyon, Mehrdad J. Gangeh, Roxana Vlad, Michael C. Kolios, and Gregory J. Czarnota
  • Fluorochrome-labeled inhibitors of caspases : expedient in vitro and in vivo markers of apoptotic cells for rapid cytometric analysis / Zbigniew Darzynkiewicz, Hong Zhao, H. Dorota Halicka, Piotr Pozarowski, and Brian Lee
  • Fast-halo assay for the detection of DNA damage / Piero Sestili, Cinzia Calcabrini, Anna Rita Diaz, Carmela Fimognari, and Vilberto Stocchi
  • Rapid detection of bacterial susceptibility or resistance to quinolones / Fátima Otero, Rebeca Santiso, María Tamayo, Germán Bou, Jaime Gosálvez, and José Luis Fernández
  • Rapid detection of apoptosis in cultured mammalian cells / Igor Kudryavtsev, Maria Serebryakova, Liudmila Solovjeva, Maria Svetlova, and Denis Firsanov
  • Quick detection of DNase II-type breaks in formalin-fixed tissue sections / Candace L. Minchew and Vladimir V. Didenko
  • Express γ-H2AX immunocytochemical detection of DNA damage / Nate Hopp, Jodi Hagen, Birte Aggeler, and Alexander E. Kalyuzhny
  • Rapid detection of γ-H2AX by flow cytometry in cultured mammalian cells / Denis Firsanov, Liudmila Solovjeva, Olga Lublinskaya, Valeriy Zenin, Igor Kudryavtsev, Maria Serebryakova, and Maria Svetlova
  • Rapid detection of DNA strand breaks in apoptotic cells by flow- and image-cytometry / Hong Zhao and Zbigniew Darzynkiewicz
  • Fast micromethod : determination of DNA integrity in cell suspensions and in solid tissues / Nevenka Bihari
  • Quantification of DNA damage and repair in mitochondrial, nuclear, and bacterial genomes by real-time PCR / Nadja Patenge
  • Zebra tail amplification : accelerated detection of apoptotic blunt-ended DNA breaks by in situ ligation / Vladimir V. Didenko
  • Twelve-gel comet assay format for quick examination of DNA damage and repair / Sergey Shaposhnikov and Andrew Collins
  • Immunofluorescence analysis of γ-H2AX foci in mammalian fibroblasts at different phases of the cell cycle / Liudmila Solovjeva, Denis Firsanov, Nadezhda Pleskach, and Maria Svetlova
  • RAPD-PCR as a rapid approach for the evaluation of genotoxin-induced damage to bacterial DNA / Rosanna Tofalo and Aldo Corsetti
  • Rapid detection of γ-H2Av foci in ex vivo MMS-treated Drosophila imaginal discs / Varandt Y. Khodaverdian and Mitch McVey.

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