Site-specific recombinases : methods and protocols / edited by Nikolai Eroshenko.
This books aims to provide an up-to-date guide to using recombinases in the laboratory. Chapters guide the reader through construction of transgenic animals, recombinase-mediated cassette exchange (RMCE), spatial control of recombinase expression and in vivo delivery approaches, immunohistochemistry...
Saved in:
| Online Access: |
Full Text (via Springer) |
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| Other Authors: | |
| Format: | Electronic eBook |
| Language: | English |
| Published: |
New York :
Humana Press : Springer,
©2017.
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| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 1642. |
| Subjects: |
| Summary: | This books aims to provide an up-to-date guide to using recombinases in the laboratory. Chapters guide the reader through construction of transgenic animals, recombinase-mediated cassette exchange (RMCE), spatial control of recombinase expression and in vivo delivery approaches, immunohistochemistry and fluorescence-based phenotyping methods, biochemical methods, and recombinase-based production of minimal DNA vectors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Site-Specific Recombinases: Methods and Protocols aims to serve as a valuable resource for working with recombinases, and inspires further creativity in the field. |
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| Physical Description: | 1 online resource (xi, 342 pages) : illustrations (some color) |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: | 9781493971695 1493971697 1493971670 9781493971671 |
| ISSN: | 1064-3745 ; |
| Source of Description, Etc. Note: | Online resource; title from PDF title page (SpringerProtocol, viewed August 17, 2017) |