Site-specific recombinases : methods and protocols / edited by Nikolai Eroshenko.

This books aims to provide an up-to-date guide to using recombinases in the laboratory. Chapters guide the reader through construction of transgenic animals, recombinase-mediated cassette exchange (RMCE), spatial control of recombinase expression and in vivo delivery approaches, immunohistochemistry...

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Bibliographic Details
Online Access: Full Text (via Springer)
Other Authors: Eroshenko, Nikolai (Editor)
Format: Electronic eBook
Language:English
Published: New York : Humana Press : Springer, ©2017.
Series:Methods in molecular biology (Clifton, N.J.) ; v. 1642.
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Table of Contents:
  • Generating genetically modified mice : a decision guide / Ivo J. Huijbers
  • Direct generation of conditional alleles using CRISPR/Cas9 in mouse zygotes / Colin E.J. Pritchard, Lona J. Kroese, and Ivo J. Huijbers
  • Building cre knockin rat lines using CRISPR/Cas9 / Yuanwu Ma, Lianfeng Zhang, and Xingxu Huang
  • Dual recombinase-mediated cassette exchange by tyrosine site-specific recombinases / Eugenia Voziyanova, Rachelle P. Anderson, and Yuri Voziyanov
  • Use of the DICE (dual integrase cassette exchange) system / Alfonso P. Farruggio, Mital S. Bhakta, and Michele P. Calos
  • Ligand-controlled site-specific recombination in zebrafish / Avinash Chekuru, Veronika Kuscha, Stefan Hans, and Michael Brand
  • Injection-based delivery of cell-permeable peptide-tagged cre / Wei-Ming Chien, Yonggang Liu, Ana A. Dinca, and Michael T. Chin
  • Viral delivery of GFP-dependent recombinases to the mouse brain / Jonathan C.Y. Tang, Stephanie Rudolph, and Constance L. Cepko
  • Recombinase-mediated cassette exchange using adenoviral vectors / Andreas F. Kolb, Christopher Knowles, Patrikas Pultinevicius, Jennifer A. Harbottle, Linda Petrie, Claire Robinson, and David A. Sorrell
  • Marker removal in transgenic plants using cre recombinase delivered with potato virus X / Lilya Kopertekh and Joachim Schiemann
  • Nanoparticle-mediated recombinase delivery into maize / Susana Martin-Ortigosa, Brian G. Trewyn, and Kan Wang
  • Immunohistochemical procedures for characterizing the retinal expression patterns of cre driver mouse lines / Qi Lu and Zhuo-Hua Pan
  • FLPing genes on and off in Drosophila / Bonnie M. Weasner, Jinjin Zhu, and Justin P. Kumar
  • Imaging neural architecture in brainbow samples / Douglas H. Roossien and Dawen Cai
  • Purification and in vitro characterization of zinc finger recombinases / Femi J. Olorunniji, Susan J. Rosser, and W. Marshall Stark
  • Preparing mate-paired illumina libraries using cre recombinase / Ze Peng, Nandita Nath, Zhiying Zhao, Jeff L. Froula, Jan-Fang Cheng, and Feng Chen
  • Preparing fosmid mate-paired libraries using Cre-LoxP recombination / Ze Peng, Jeff L. Froula, and Jan-Fang Cheng
  • Using purified tyrosine site-specific recombinases in vitro to rapidly construct and diversify metabolic pathways / Wei Liu, Laura R. Tuck, Jon Marles Wright, and Yizhi Cai
  • Multipart DNA assembly using site-specific recombinases from the large serine integrase family / Femi J. Olorunniji, Christine Merrick, Susan J. Rosser, Margaret C.M. Smith, W. Marshall Stark, and Sean D. Colloms
  • Production of minicircle DNA vectors using site-specific recombinases / Nafiseh Sanei Ata-abadi, Naeimeh Rezaei, Kianoush Dormiani, and Mohammad Hossein Nasr-Esfahani.