Nanotoxicity : methods and protocols / edited by Qunwei Zhang.
This volume explores the latest techniques used to investigate nanotoxicity. The protocols covered in this book range from routine nanotoxicity assays to genomic and proteomic approaches in vivo and in vitro. The chapters discuss specific topics such as preparation and utilization of 3D human liver...
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Full Text (via Springer) |
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| Format: | Electronic eBook |
| Language: | English |
| Published: |
New York, NY :
Humana Press : Springer,
[2019]
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| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 1894. Springer protocols (Series) |
| Subjects: |
MARC
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| 264 | 1 | |a New York, NY : |b Humana Press : |b Springer, |c [2019] | |
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| 490 | 1 | |a Methods in molecular biology, |x 1940-6029 ; |v 1894 | |
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| 504 | |a Includes bibliographical references and index. | ||
| 588 | 0 | |a Print version record. | |
| 505 | 0 | 0 | |t In vitro methods for assessing nanoparticle toxicity / |r Dustin T. Savage, J. Zach Hilt, and Thomas D. Dziubla -- |t Methods for assessing mast cell responses to engineered nanomaterial exposure / |r Nasser B. Alsaleh and Jared M. Brown -- |t Preparation and u tilization of a 3D human liver microtissue model for nanotoxicological assessment / |r Ali Kermanizadeh -- |t Deriving neural cells from pluripotent stem cells for nanotoxicity testing / |r Yiling Hong, Nymph Chan, and Aynun N. Begum -- |t Methods for evaluation of ovarian granulosa cells with exposure to nanoparticles / |r Jin Liu and Wenchang Zhang -- |t In vitro approaches for assessing the genotoxicity of nanomaterials / |r Maria Dusinska, Espen Mariussen, Elise Rundeþn-Pran, Alexandra Misci Hudecova, Elisabeth Elje, Alena Kazimirova, Naouale El Yamani, Nils Dommershausen, Julian Tharmann, Dagmar Fieblinger, Frank Herzberg, Andreas Luch, and Andrea Haase -- |t Measurement of nanoparticle-induced mitochondrial membrane potential alterations / |r Salik Hussain -- |t Application of gelatin zymography in nanotoxicity research / |r Yue Zhang, Rong Wan, Qunwei Zhang, and Yiqun Mo -- |t Determination of phosphorylated histone H2AX in nanoparticle-induced genotoxic studies / |r Rong Wan, Yiqun Mo, Ruirui Tong, Meiqin Gao, and Qunwei Zhang -- |t Analysis of nanomaterial toxicity by western blot / |r Gao Long, Yiqun Mo, Qunwei Zhang, and Mizu Jiang -- |t Quantification of mitochondrial genome transcription in male reproductive cells by real-time PCR / |r Cheng Xu, Qian Liu, Jin Xu, and Aihua Gu -- |t DNA methylation analysis / |r Lingfang Feng and Jianlin Lou -- |t Evaluation of Nrf2 with exposure to nanoparticles / |r Fuli Zheng and Huangyuan Li -- |t Application of electron microscopes in nanotoxicity assessment / Junzhe Zhang, Xiao He, and Michael T. Tseng -- Metabolic fingerprinting of bacteria exposed to nanomaterials, using online databases, NMR, and high-resolution mass spectrometry / |r Theodoros G. Chatzimitakos and Constantine D. Stalikas -- |t Methods to quantify nanomaterial association with, and distribution across, the blood-brain barrier in vivo / |r Robert A. Yokel -- |t Evaluation of genotoxicity of nanoparticles in mouse models / |r Sameera Nallanthighal and Ramune Reliene -- |t Evaluation of pulmonary toxicity of nanoparticles by bronchoalveolar lavage / |r Yiqun Mo, Yue Zhang, and Qunwei Zhang -- |t Pulmonary function testing in animals / |r Gary W. Hoyle, Connie F. Schlueter, and Sadiatu Musah -- |t Nanotoxicity assessment using embryonic zebrafish / |r Eduard Dumitrescu, Kenneth Wallace, and Silvana Andreescu -- |t Application of Daphnia magna for nanoecotoxicity study / |r Zhizhen Xu, Yingying Liu, and Yuqian Wang -- |t Overview of methods of nanoparticle exposure assessment / |r Peng Zhao and Yuanbao Zhang. |
| 520 | |a This volume explores the latest techniques used to investigate nanotoxicity. The protocols covered in this book range from routine nanotoxicity assays to genomic and proteomic approaches in vivo and in vitro. The chapters discuss specific topics such as preparation and utilization of 3D human liver microtissue models; assessment of ovarian granulosa cells with exposure to nanoparticles; DNA methylation analysis; evaluation of genotoxicity of nanoparticles in mouse models; and studying nanotoxicity using embryonic zebrafish. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. | ||
| 650 | 0 | |a Nanostructured materials |x Toxicology |v Laboratory manuals. | |
| 650 | 0 | |a Nanotechnology |v Laboratory manuals. | |
| 650 | 7 | |a Nanotechnology |2 fast | |
| 655 | 7 | |a Laboratory manuals |2 fast | |
| 700 | 1 | |a Zhang, Qunwei, |e editor. | |
| 776 | 0 | 8 | |i Print version: |t Nanotoxicity. |d New York, N.Y : Springer, ©2019 |z 9781493989157 |
| 830 | 0 | |a Methods in molecular biology (Clifton, N.J.) ; |v v. 1894. |x 1064-3745 | |
| 830 | 0 | |a Springer protocols (Series) | |
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