Development of Chemical and Metabolite Sensors for <i>Rhodococcus opacus</i> PD630 [electronic resource]
Rhodococcus Opacus; Lignin; Promoter; Sensor; Nitrogen; Aromatic.
Saved in:
| Online Access: |
Online Access (via OSTI) |
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| Corporate Author: | |
| Format: | Government Document Electronic eBook |
| Language: | English |
| Published: |
Washington, D.C. : Oak Ridge, Tenn. :
United States. Department of Energy. Office of Science ; distributed by the Office of Scientific and Technical Information, U.S. Department of Energy,
2017.
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| Subjects: |
MARC
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| 245 | 0 | 0 | |a Development of Chemical and Metabolite Sensors for <i>Rhodococcus opacus</i> PD630 |h [electronic resource] |
| 260 | |a Washington, D.C. : |b United States. Department of Energy. Office of Science ; |a Oak Ridge, Tenn. : |b distributed by the Office of Scientific and Technical Information, U.S. Department of Energy, |c 2017. | ||
| 300 | |a p. 1973-1978 : |b digital, PDF file. | ||
| 336 | |a text |b txt |2 rdacontent. | ||
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| 338 | |a online resource |b cr |2 rdacarrier. | ||
| 500 | |a Published through SciTech Connect. | ||
| 500 | |a 07/26/2017. | ||
| 500 | |a ACS Synthetic Biology 6 10 ISSN 2161-5063 AM. | ||
| 500 | |a Drew M. DeLorenzo; William R. Henson; Tae Seok Moon. | ||
| 520 | 3 | |a Rhodococcus opacus PD630 is a non-model, gram positive bacterium that possesses desirable traits for biomass conversion, including consumption capabilities for lignocellulose-based sugars and toxic lignin-derived aromatic compounds, significant triacylglycerol accumulation, relatively rapid growth rate, and genetic tractability. However, few genetic elements have been directly characterized in R. opacus, limiting its application for lignocellulose bioconversion. Here, we report the characterization and development of genetic tools for tunable gene expression in R. opacus, including: 1) six fluorescent reporters for quantifying promoter output, 2) three chemically inducible promoters for variable gene expression, and 3) two classes of metabolite sensors derived from native R. opacus promoters that detect nitrogen levels or aromatic compounds. Using these tools, we also provide insights into native aromatic consumption pathways in R. opacus. Overall, this work expands the ability to control and characterize gene expression in R. opacus for future lignocellulose-based fuel and chemical production. | |
| 520 | 0 | |a Rhodococcus Opacus; Lignin; Promoter; Sensor; Nitrogen; Aromatic. | |
| 536 | |b SC0012705. | ||
| 650 | 7 | |a Biomass Fuels. |2 edbsc. | |
| 710 | 2 | |a Washington University (Saint Louis, Mo. |b ). |4 res. | |
| 710 | 1 | |a United States. |b Department of Energy. |b Office of Science. |4 spn. | |
| 710 | 1 | |a United States. |b Department of Energy. |b Office of Scientific and Technical Information. |4 dst. | |
| 856 | 4 | 0 | |u http://www.osti.gov/scitech/biblio/1417982 |z Online Access (via OSTI) |
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| 952 | f | f | |p Can circulate |a University of Colorado Boulder |b Online |c Online |d Online |e E 1.99:1417982 |h Superintendent of Documents classification |i web |n 1 |