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|a Co-hydrolysis of hydrothermal and dilute acid pretreated populus slurries to support development of a high-throughput pretreatment system
|h [electronic resource]
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| 260 |
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|a Washington, D.C. :
|b United States. Department of Energy. Office of Science ;
|a Oak Ridge, Tenn. :
|b Distributed by the Office of Scientific and Technical Information, U.S. Department of Energy,
|c 2011.
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| 300 |
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|a Size: Article No. 19 :
|b digital, PDF file.
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|a text
|b txt
|2 rdacontent.
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|a Published through Scitech Connect.
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|a 01/01/2011.
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|a "Journal ID: ISSN 1754-6834."
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|a "Other: PII: 1754-6834-4-19."
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|a Studer, Michael H. ; Brethauer, Simone ; DeMartini, Jaclyn D. ; McKenzie, Heather L. ; Wyman, Charles E. ;
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|a Background: The BioEnergy Science Center (BESC) developed a high-throughput screening method to rapidly identify low-recalcitrance biomass variants. Because the customary separation and analysis of liquid and solids between pretreatment and enzymatic hydrolysis used in conventional analyses is slow, labor-intensive and very difficult to automate, a streamlined approach we term ?co-hydrolysis? was developed. In this method, the solids and liquid in the pretreated biomass slurry are not separated, but instead hydrolysis is performed by adding enzymes to the whole pretreated slurry. The effects of pretreatment method, severity and solids loading on co-hydrolysis performance were investigated. Results: For hydrothermal pretreatment at solids concentrations of 0.5 to 2%, high enzyme protein loadings of about 100 mg/g of substrate (glucan plus xylan) in the original poplar wood achieved glucose and xylose yields for co-hydrolysis that were comparable with those for washed solids. In addition, although poplar wood sugar yields from co-hydrolysis at 2% solids concentrations fell short of those from hydrolysis of washed solids after dilute sulfuric acid pretreatment even at high enzyme loadings, pretreatment at 0.5% solids concentrations resulted in similar yields for all but the lowest enzyme loading. Conclusions: Overall, the influence of severity on susceptibility of pretreated substrates to enzymatic hydrolysis was clearly discernable, showing co-hydrolysis to be a viable approach for identifying plant-pretreatment-enzyme combinations with substantial advantages for sugar production.
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|b AC05-00OR22725.
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| 650 |
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7 |
|a 09 biomass fuels
|2 local.
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| 650 |
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7 |
|a 59 basic biological sciences
|2 local.
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| 650 |
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|a Biotechnology & applied microbiology
|2 local.
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| 650 |
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|a Energy & fuels
|2 local.
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| 710 |
2 |
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|a United States. Department of Energy. Office of Science.
|4 spn.
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| 710 |
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|a United States.
|b Department of Energy.
|b Office of Scientific and Technical Information
|4 dst.
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| 856 |
4 |
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|u https://www.osti.gov/servlets/purl/1626655
|z Full Text (via OSTI)
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|b 04-21-21
|c 04-21-21
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|a University of Colorado Boulder
|b Online
|c Online
|d Online
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