Mutations in a plastid-localized elongation factor G alter early stages of plastid development in Arabidopsis thaliana [electronic resource]
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Full Text (via OSTI) |
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| Format: | Electronic eBook |
| Language: | English |
| Published: |
Washington, D.C. : Oak Ridge, Tenn. :
United States. Department of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Department of Energy,
2007.
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| Subjects: |
| Abstract: | Background: Proper development of plastids in embryo and seedling tissues is critical for plantdevelopment. During germination, plastids develop to perform many critical functions that arenecessary to establish the seedling for further growth. A growing body of work has demonstratedthat components of the plastid transcription and translation machinery must be present andfunctional to establish the organelle upon germination.Results: We have identified Arabidopsis thaliana mutants in a gene that encodes a plastid-targetedelongation factor G (SCO1) that is essential for plastid development during embryogenesis sincetwo T-DNA insertion mutations in the coding sequence (sco1-2 and sco1-3) result in an embryolethal phenotype. In addition, a point mutation allele (sco1-1) and an allele with a T-DNA insertionin the promoter (sco1-4) of SCO1 display conditional seedling-lethal phenotypes. Seedlings of thesealleles exhibit cotyledon and hypocotyl albinism due to improper chloroplast development, andnormally die shortly after germination. However, when germinated on media supplemented withsucrose, the mutant plants can produce photosynthetically-active green leaves from the apicalmeristem.Conclusion: The developmental stage-specific phenotype of the conditional-lethal sco1 allelesreveals differences in chloroplast formation during seedling germination compared to chloroplastdifferentiation in cells derived from the shoot apical meristem. Our identification of embryo-lethalmutant alleles in the Arabidopsis elongation factor G indicates that SCO1 is essential for plantgrowth, consistent with its predicted role in chloroplast protein translation. |
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| Item Description: | Published through Scitech Connect. 01/01/2007. "Journal ID: ISSN 1471-2229." "Other: PII: 1471-2229-7-37." Ruppel, Nicholas J. ; Hangarter, Roger P. ; |
| Physical Description: | Size: Article No. 37 : digital, PDF file. |