Gene knockout protocols [electronic resource]

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Bibliographic Details
Online Access: Online Access
Other Authors: Kühn, Ralf, Wurst, Wolfgang, 1955-
Format: Electronic eBook
Language:English
Published: New York, NY : Humana Press, ©2009.
Edition:2nd ed. /
Series:Methods in molecular biology (Clifton, N.J.) ; v. 530.
Springer protocols (Series)
Subjects:

MARC

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245 0 0 |a Gene knockout protocols  |h [electronic resource] 
250 |a 2nd ed. /  |b edited by Ralf Khün, Wolfgang Wurst. 
260 |a New York, NY :  |b Humana Press,  |c ©2009. 
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490 1 |a Methods in molecular biology,  |x 1064-3745 ;  |v 530. 
490 1 |a Springer protocols. 
504 |a Includes bibliographical references and index. 
505 0 |a Overview on mouse mutagenesis / Ralf Kühn and Wolfgang Wurst -- Construction of gene-targeting vectors by recombineering / Song-Choon Lee, Wei Wang, and Pentao Liu -- Gene-trap vectors and mutagenesis / Silke De-Zolt [and others] -- Chromosome engineering in ES cells / Louise vander Weyden, Charles Shaw-Smith, and Allan Bradley -- Gene modification in embryonic stem cells by single-stranded DNA oligonucleotides / Marieke Aarts [and others] -- Generation of shRNA transgenic mice / Christiane Hitz [and others] -- Mutagenesis of mouse embryonic stem cells with ethylmethanesulfonate / Robert Munroe and John Schimenti -- Gene targeting in mouse embryonic stem cells / Lino Tessarollo [and others] -- Manipulating mouse embryonic stem cells / Eileen Southon and Lino Tessarollo -- ES cell line establishment / Heidrun Kern and Branko Zevnik -- Generation of double-knockout embryonic stem cells / Eva Wielders, Marleen Dekker, and Hein te Riele -- Differentiation analysis of pluripotent mouse embryonic stem (ES) cells in vitro / Insa S. Schroeder [and others] -- Cloning of ES cells and mice by nuclear transfer / Sayaka Wakayama, Satoshi Kishigami, and Teruhiko Wakayama -- Isolation, microinjection, and transfer of mouse blastocysts / Susan W. Reid and Lino Tessarollo -- Aggregation chimeras : combining ES cells, diploid, and tetraploid embryos / Mika Tanaka [and others] -- VelociMouse : fully ES cell-derived F0-generation mice obtained from the injection of ES cells into eight-cell-stage embryos / Thomas M. DeChiara [and others] -- Generation of Cre recombinase-expressing transgenic mice using bacterial artificial chromosomes / Jan Rodriguez Parkitna, David Engblom, and Günther Schütz -- Inducible Cre mice / Susanne Feil, Nadejda Valtcheva, and Robert Feil -- Creation and use of a Cre recombinase transgenic database / Andras Nagy, Lynn Mar, and Graham Watts -- Transposon mutagenesis in mice / David A. Largaespada -- Lentiviral transgenesis / Alexander Pfeifer and Andreas Hofmann -- Sperm cryopreservation and in vitro fertilization / Susan Marschall, Auke Boersma, and Martin Hrabé de Angelis -- Influence of genetic background on genetically engineered mouse phenotypes / Thomas Doetschman -- Pathologic phenotyping of mutant mice / Roderick T. Bronson -- Systemic first-line phenotyping / Valérie Gailus-Durner [and others] 
588 0 |a Print version record. 
650 0 |a Gene targeting. 
650 0 |a Transgenic mice. 
700 1 |a Kühn, Ralf. 
700 1 |a Wurst, Wolfgang,  |d 1955- 
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