Gene knockout protocols [electronic resource]
Saved in:
Online Access: |
Online Access |
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Other Authors: | , |
Format: | Electronic eBook |
Language: | English |
Published: |
New York, NY :
Humana Press,
©2009.
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Edition: | 2nd ed. / |
Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 530. Springer protocols (Series) |
Subjects: |
MARC
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245 | 0 | 0 | |a Gene knockout protocols |h [electronic resource] |
250 | |a 2nd ed. / |b edited by Ralf Khün, Wolfgang Wurst. | ||
260 | |a New York, NY : |b Humana Press, |c ©2009. | ||
300 | |a 1 online resource (xvi, 516 pages, [4] pages of plates) : |b illustrations (some color) | ||
336 | |a text |b txt |2 rdacontent. | ||
337 | |a computer |b c |2 rdamedia. | ||
338 | |a online resource |b cr |2 rdacarrier. | ||
490 | 1 | |a Methods in molecular biology, |x 1064-3745 ; |v 530. | |
490 | 1 | |a Springer protocols. | |
504 | |a Includes bibliographical references and index. | ||
505 | 0 | |a Overview on mouse mutagenesis / Ralf Kühn and Wolfgang Wurst -- Construction of gene-targeting vectors by recombineering / Song-Choon Lee, Wei Wang, and Pentao Liu -- Gene-trap vectors and mutagenesis / Silke De-Zolt [and others] -- Chromosome engineering in ES cells / Louise vander Weyden, Charles Shaw-Smith, and Allan Bradley -- Gene modification in embryonic stem cells by single-stranded DNA oligonucleotides / Marieke Aarts [and others] -- Generation of shRNA transgenic mice / Christiane Hitz [and others] -- Mutagenesis of mouse embryonic stem cells with ethylmethanesulfonate / Robert Munroe and John Schimenti -- Gene targeting in mouse embryonic stem cells / Lino Tessarollo [and others] -- Manipulating mouse embryonic stem cells / Eileen Southon and Lino Tessarollo -- ES cell line establishment / Heidrun Kern and Branko Zevnik -- Generation of double-knockout embryonic stem cells / Eva Wielders, Marleen Dekker, and Hein te Riele -- Differentiation analysis of pluripotent mouse embryonic stem (ES) cells in vitro / Insa S. Schroeder [and others] -- Cloning of ES cells and mice by nuclear transfer / Sayaka Wakayama, Satoshi Kishigami, and Teruhiko Wakayama -- Isolation, microinjection, and transfer of mouse blastocysts / Susan W. Reid and Lino Tessarollo -- Aggregation chimeras : combining ES cells, diploid, and tetraploid embryos / Mika Tanaka [and others] -- VelociMouse : fully ES cell-derived F0-generation mice obtained from the injection of ES cells into eight-cell-stage embryos / Thomas M. DeChiara [and others] -- Generation of Cre recombinase-expressing transgenic mice using bacterial artificial chromosomes / Jan Rodriguez Parkitna, David Engblom, and Günther Schütz -- Inducible Cre mice / Susanne Feil, Nadejda Valtcheva, and Robert Feil -- Creation and use of a Cre recombinase transgenic database / Andras Nagy, Lynn Mar, and Graham Watts -- Transposon mutagenesis in mice / David A. Largaespada -- Lentiviral transgenesis / Alexander Pfeifer and Andreas Hofmann -- Sperm cryopreservation and in vitro fertilization / Susan Marschall, Auke Boersma, and Martin Hrabé de Angelis -- Influence of genetic background on genetically engineered mouse phenotypes / Thomas Doetschman -- Pathologic phenotyping of mutant mice / Roderick T. Bronson -- Systemic first-line phenotyping / Valérie Gailus-Durner [and others] | |
588 | 0 | |a Print version record. | |
650 | 0 | |a Gene targeting. | |
650 | 0 | |a Transgenic mice. | |
700 | 1 | |a Kühn, Ralf. | |
700 | 1 | |a Wurst, Wolfgang, |d 1955- | |
776 | 0 | 8 | |i Print version: |t Gene knockout protocols. |b 2nd ed. |d New York, NY : Humana Press, ©2009 |z 9781934115268 |w (DLC) 2009920951 |w (OCoLC)276226992. |
830 | 0 | |a Methods in molecular biology (Clifton, N.J.) ; |v v. 530. | |
830 | 0 | |a Springer protocols (Series) | |
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956 | |a Springer protocols | ||
956 | |b Springer Complete Protocols | ||
956 | |b Springer Complete eBooks Contemporary 2005+ | ||
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952 | f | f | |p Can circulate |a University of Colorado Boulder |b Online |c Online |d Online |e QH442.3 |h Library of Congress classification |i web |n 1 |