Recombinant proteins from plants : methods and protocols / edited by Jacqueline MacDonald, Igor Kolotilin, Rima Menassa.

This volume provides up-to-date scientific achievements from the world's top researchers. Recombinant Proteins from Plants: Methods and Protocols, Second Edition guides readers through protocolsfor use with a variety of plant expression systems. Various aspects of production are covered includi...

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Bibliographic Details
Other Authors: MacDonald, Jacqueline (Of Agriculture and Agri-Food Canada) (Editor), Kolotilin, Igor (Editor), Menassa, Rima (Editor)
Format: Book
Language:English
Published: New York : Humana Press, [2016]
Edition:Second edition.
Series:Methods in molecular biology (Clifton, N.J.) ; v. 1385.
Springer protocols (Series)
Subjects:
Table of Contents:
  • Chloroplast-based expression of recombinant proteins by Gateway® cloning technology / Johanna Gottschamel and Andreas Lössl
  • Multigene engineering in rice using high-capacity Agrobacterium tumefaciens BIBAC vectors / Ruifeng He
  • Virus-derived vectors for the expression of multiple proteins in plants / Pooja Saxena [and others]
  • Transient protein expression by agroinfiltration in lettuce / Qiang Chen [and others]
  • Production of recombinant proteins in the chloroplast of the green alga Chlamydomonas reinhardtii / Daniel Guzmán-Zapata [and others]
  • Efficient, antibiotic marker-free transformation of a dicot and a monocot crop with glutamate 1-semialdehyde aminotransferase selectable marker genes / Nicoletta Ferradini [and others]
  • Transient expression of mammalian genes in N. benthamiana to modulate N-glycosylation / Alexandra Castilho and Herta Steinkellner
  • Companion protease inhibitors for the in situ protection of recombinant proteins in plants / Stéphanie Robert [and others]
  • Production of recombinant cholera toxin B subunit in Nicotiana benthamiana using GENEWARE® tobacco mosaic virus vector / Lauren Moore, Krystal Hamorsky, and Nobuyuki Matoba
  • Total soluble protein extraction for improved proteomic analysis of transgenic rice plant roots / Manish L. Raorane, Joan O. Narciso, and Ajay Kohli
  • Temporary immersion bioreactors for the contained production of recombinant proteins in transplastomic plants / Sherwin Barretto, Franck Michoux, and Peter J. Nixon
  • Plant cell-based recombinant antibody manufacturing with a 200 L orbitally shaken disposable bioreactor / Nicole Raven, Stefan Schillberg, and Stefan Rasche
  • Liquid-liquid phase separation of oil bodies from seeds / Cory L. Nykiforuk
  • Continuous flow separation of hydrophobin fusion proteins from plant cell culture extract / Lauri J. Reuter, Andrew J. Conley, and Jussi J. Joensuu
  • Molecular analyses of transgenic plants / Kurniawan Rudi Trijatmiko [and others]
  • Analysis of recombinant proteins in transgenic rice seeds : identity, localization, tolerance to digestion, and plant stress response / Yuhya Wakasa and Fumio Takaiwa
  • Real-time PCR-based quantitation method for the genetically modified soybean line GTS 40-3-2 / Kazumi Kitta, Reona Takabatake, and Junichi Mano
  • Standardized lepidopteran bioassay to investigate the bioactivity of insecticidal proteins produced in transgenic crops / Gerson Graser and Frederick S. Walters
  • Antibody-mediated pathogen resistance in plants / Dieter Peschen, Stefan Schillberg, and Rainer Fischer.